Biophoton
Encyclopedia
A biophoton synonymous with ultraweak photon emission, low-level biological chemiluminescence, ultraweak bioluminescence, dark luminescence and other similar terms, is a photon
Photon
In physics, a photon is an elementary particle, the quantum of the electromagnetic interaction and the basic unit of light and all other forms of electromagnetic radiation. It is also the force carrier for the electromagnetic force...

 of light
Light
Light or visible light is electromagnetic radiation that is visible to the human eye, and is responsible for the sense of sight. Visible light has wavelength in a range from about 380 nanometres to about 740 nm, with a frequency range of about 405 THz to 790 THz...

 emitted from a biological system
Life
Life is a characteristic that distinguishes objects that have signaling and self-sustaining processes from those that do not, either because such functions have ceased , or else because they lack such functions and are classified as inanimate...

 and detected by biological probes as part of the general weak electromagnetic radiation
Electromagnetic radiation
Electromagnetic radiation is a form of energy that exhibits wave-like behavior as it travels through space...

 of living biological cells. Biophotons and their study should not be confused with bioluminescence
Bioluminescence
Bioluminescence is the production and emission of light by a living organism. Its name is a hybrid word, originating from the Greek bios for "living" and the Latin lumen "light". Bioluminescence is a naturally occurring form of chemiluminescence where energy is released by a chemical reaction in...

, a term generally reserved for higher intensity luciferin
Luciferin
Luciferins are a class of light-emitting biological pigments found in organisms that cause bioluminescence...

/luciferase
Luciferase
Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. One famous example is the firefly luciferase from the firefly Photinus pyralis. "Firefly luciferase" as a laboratory reagent usually refers to P...

 systems.

Biophotonics
Biophotonics
The term biophotonics denotes a combination of biology and photonics, with photonics being the science and technology of generation, manipulation, and detection of photons, quantum units of light. Photonics is related to electronics in that it is believed that photons will play a similar central...

 is the study, research and applications of photons in their interactions within and on biological systems. Topics of research pertain more generally to basic questions of biophysics
Biophysics
Biophysics is an interdisciplinary science that uses the methods of physical science to study biological systems. Studies included under the branches of biophysics span all levels of biological organization, from the molecular scale to whole organisms and ecosystems...

 and related subjects - for example, the regulation of biological functions, cell growth
Cell growth
The term cell growth is used in the contexts of cell development and cell division . When used in the context of cell division, it refers to growth of cell populations, where one cell grows and divides to produce two "daughter cells"...

 and differentiation, connections to so-called delayed luminescence
Luminescence
Luminescence is emission of light by a substance not resulting from heat; it is thus a form of cold body radiation. It can be caused by chemical reactions, electrical energy, subatomic motions, or stress on a crystal. This distinguishes luminescence from incandescence, which is light emitted by a...

, and spectral emissions in supermolecular
Supramolecular chemistry
Supramolecular chemistry refers to the area of chemistry beyond the molecules and focuses on the chemical systems made up of a discrete number of assembled molecular subunits or components...

 processes in living tissues, etc.

The typical detected magnitude
Magnitude (mathematics)
The magnitude of an object in mathematics is its size: a property by which it can be compared as larger or smaller than other objects of the same kind; in technical terms, an ordering of the class of objects to which it belongs....

 of "biophotons" in the visible and ultraviolet spectrum
Electromagnetic spectrum
The electromagnetic spectrum is the range of all possible frequencies of electromagnetic radiation. The "electromagnetic spectrum" of an object is the characteristic distribution of electromagnetic radiation emitted or absorbed by that particular object....

 ranges from a few up to several hundred photons per second per square centimeter of surface area, much weaker than in the openly visible and well-researched phenomenon of normal bioluminescence, but stronger than in the thermal, or black body radiation that so-called perfect black bodies demonstrate. The detection of these photons has been made possible (and easier) by the development of more sensitive photomultiplier
Photomultiplier
Photomultiplier tubes , members of the class of vacuum tubes, and more specifically phototubes, are extremely sensitive detectors of light in the ultraviolet, visible, and near-infrared ranges of the electromagnetic spectrum...

 tubes and associated electronic equipment.

Biophotons were employed by the Stalin regime to diagnose cancer
Cancer
Cancer , known medically as a malignant neoplasm, is a large group of different diseases, all involving unregulated cell growth. In cancer, cells divide and grow uncontrollably, forming malignant tumors, and invade nearby parts of the body. The cancer may also spread to more distant parts of the...

, and their discoverer, Alexander Gurwitsch
Alexander Gurwitsch
Alexander Gavrilovich Gurwitsch was a Russian and Soviet biologist and medical scientist who originated the morphogenetic field theory and discovered the biophoton...

 was awarded the Stalin Prize.
Various studies have indicated some potential for photon emission to be used as a diagnostic technique.

History

In the 1920s, the Russian embryologist Alexander Gurwitsch
Alexander Gurwitsch
Alexander Gavrilovich Gurwitsch was a Russian and Soviet biologist and medical scientist who originated the morphogenetic field theory and discovered the biophoton...

 reported "ultraweak" photon emissions from living tissues in the UV-range of the spectrum. He named them "mitogenetic rays" because his experiments convinced him that they had a stimulating effect on cell division
Cell division
Cell division is the process by which a parent cell divides into two or more daughter cells . Cell division is usually a small segment of a larger cell cycle. This type of cell division in eukaryotes is known as mitosis, and leaves the daughter cell capable of dividing again. The corresponding sort...

. (see Morphogenetic field
Morphogenetic field
In developmental biology, a morphogenetic field is a group of cells able to respond to discrete, localized biochemical signals leading to the development of specific morphological structures or organs. The spatial and temporal extent of the embryonic fields are dynamic, and within the field is a...

) However, the failure to replicate his findings and the fact that, though cell growth can be stimulated and directed by radiation
Electromagnetic radiation
Electromagnetic radiation is a form of energy that exhibits wave-like behavior as it travels through space...

 this is possible only at much higher amplitudes, evoked a general skepticism about Gurwitsch's work. In 1953 Irving Langmuir
Irving Langmuir
Irving Langmuir was an American chemist and physicist. His most noted publication was the famous 1919 article "The Arrangement of Electrons in Atoms and Molecules" in which, building on Gilbert N. Lewis's cubical atom theory and Walther Kossel's chemical bonding theory, he outlined his...

 dubbed Gurwitsch's ideas pathological science
Pathological science
Pathological science is the process in science in which "people are tricked into false results ... by subjective effects, wishful thinking or threshold interactions". The term was first used by Irving Langmuir, Nobel Prize-winning chemist, during a 1953 colloquium at the Knolls Research Laboratory...

.

But in the later 20th century Gurwitsch's daughter Anna, Colli, Quickenden and Inaba separately returned to the subject, referring to the phenomenon more neutrally as "dark luminescence", "low level luminescence", "ultraweak bioluminescence", or "ultraweak chemiluminescence". Their common basic hypothesis was that the phenomenon was induced from rare oxidation processes and radical
Radical (chemistry)
Radicals are atoms, molecules, or ions with unpaired electrons on an open shell configuration. Free radicals may have positive, negative, or zero charge...

 reactions
Chemical reaction
A chemical reaction is a process that leads to the transformation of one set of chemical substances to another. Chemical reactions can be either spontaneous, requiring no input of energy, or non-spontaneous, typically following the input of some type of energy, such as heat, light or electricity...

. Gurwitsch's basic observations were vindicated.

Proposed mechanism

Chemiexcitation via oxidative stress
Oxidative stress
Oxidative stress represents an imbalance between the production and manifestation of reactive oxygen species and a biological system's ability to readily detoxify the reactive intermediates or to repair the resulting damage...

 by reactive oxygen species
Reactive oxygen species
Reactive oxygen species are chemically reactive molecules containing oxygen. Examples include oxygen ions and peroxides. Reactive oxygen species are highly reactive due to the presence of unpaired valence shell electrons....

(ROS) and/or catalysis
Catalysis
Catalysis is the change in rate of a chemical reaction due to the participation of a substance called a catalyst. Unlike other reagents that participate in the chemical reaction, a catalyst is not consumed by the reaction itself. A catalyst may participate in multiple chemical transformations....

 by enzymes (i.e. peroxidase
Peroxidase
Peroxidases are a large family of enzymes that typically catalyze a reaction of the form:For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides...

, lipoxygenase
Lipoxygenase
Lipoxygenases are a family of iron-containing enzymes that catalyse the dioxygenation of polyunsaturated fatty acids in lipids containing a cis,cis-1,4- pentadiene structure. It catalyses the following reaction:...

) is a common event in the biomolecular milieu. Such reactions can lead to the formation of triplet excited species, which release photons upon returning to a lower energy level
Energy level
A quantum mechanical system or particle that is bound -- that is, confined spatially—can only take on certain discrete values of energy. This contrasts with classical particles, which can have any energy. These discrete values are called energy levels...

 in a process analogous to phosphorescence
Phosphorescence
Phosphorescence is a specific type of photoluminescence related to fluorescence. Unlike fluorescence, a phosphorescent material does not immediately re-emit the radiation it absorbs. The slower time scales of the re-emission are associated with "forbidden" energy state transitions in quantum...

. That this process is a contributing factor to spontaneous biophoton emission has been indicated by studies demonstrating that biophoton emission can be attenuated by depleting assayed tissue of antioxidants or by addition of carbonyl derivitizing agents. Further support is provided by studies indicating that emission can be increased by addition of reactive oxygen species
Reactive oxygen species
Reactive oxygen species are chemically reactive molecules containing oxygen. Examples include oxygen ions and peroxides. Reactive oxygen species are highly reactive due to the presence of unpaired valence shell electrons....

 (ROS).

Since there is visible bioluminescence
Bioluminescence
Bioluminescence is the production and emission of light by a living organism. Its name is a hybrid word, originating from the Greek bios for "living" and the Latin lumen "light". Bioluminescence is a naturally occurring form of chemiluminescence where energy is released by a chemical reaction in...

 in many bacteria
Bacteria
Bacteria are a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria have a wide range of shapes, ranging from spheres to rods and spirals...

 and other cells
Cell (biology)
The cell is the basic structural and functional unit of all known living organisms. It is the smallest unit of life that is classified as a living thing, and is often called the building block of life. The Alberts text discusses how the "cellular building blocks" move to shape developing embryos....

 it can be inferred that the (extremely small) number of photons in ultra-weak bioluminescence is a random by-product of cellular metabolism
Metabolism
Metabolism is the set of chemical reactions that happen in the cells of living organisms to sustain life. These processes allow organisms to grow and reproduce, maintain their structures, and respond to their environments. Metabolism is usually divided into two categories...

. Cellular metabolism is thought to occur in steps, each involving small energy exchanges.(See ATP
Adenosine triphosphate
Adenosine-5'-triphosphate is a multifunctional nucleoside triphosphate used in cells as a coenzyme. It is often called the "molecular unit of currency" of intracellular energy transfer. ATP transports chemical energy within cells for metabolism...

) Due to a certain degree of randomness, according to the laws of thermodynamics
Thermodynamics
Thermodynamics is a physical science that studies the effects on material bodies, and on radiation in regions of space, of transfer of heat and of work done on or by the bodies or radiation...

 (or statistical mechanics
Statistical mechanics
Statistical mechanics or statistical thermodynamicsThe terms statistical mechanics and statistical thermodynamics are used interchangeably...

), it must be expected that some irregular steps will occasionally occur, "outlying states" in which, due to physiochemical energy imbalance, a photon is emitted.

Statistical mechanics
Statistical mechanics
Statistical mechanics or statistical thermodynamicsThe terms statistical mechanics and statistical thermodynamics are used interchangeably...

 in modern biology
Biology
Biology is a natural science concerned with the study of life and living organisms, including their structure, function, growth, origin, evolution, distribution, and taxonomy. Biology is a vast subject containing many subdivisions, topics, and disciplines...

 often favours an ensemble model of systems due to the large numbers of interacting molecules, etc. In chaos theory
Chaos theory
Chaos theory is a field of study in mathematics, with applications in several disciplines including physics, economics, biology, and philosophy. Chaos theory studies the behavior of dynamical systems that are highly sensitive to initial conditions, an effect which is popularly referred to as the...

, for example, it is often suggested that the apparent randomness of systems is due to a lack of understanding of the larger system of which the given system is a component. This has led many who deal with large systems to employ statistics to explain seemingly random events as outlying effects in probability distribution
Probability distribution
In probability theory, a probability mass, probability density, or probability distribution is a function that describes the probability of a random variable taking certain values....

s.

Hypothesized involvement in cellular communication

In the 1970s the then assistant professor Fritz-Albert Popp
Fritz-Albert Popp
- Biography :Fritz-Albert Popp is a German researcher in biophysics, particularly in biophotonics, a branch of quantum biology dealing with the analysis of single photons emitted from living organisms...

, and his research group, at the University of Marburg (Germany
Germany
Germany , officially the Federal Republic of Germany , is a federal parliamentary republic in Europe. The country consists of 16 states while the capital and largest city is Berlin. Germany covers an area of 357,021 km2 and has a largely temperate seasonal climate...

) showed that the spectral distribution of the emission fell over a wide range of wavelengths, from 200 to 800 nm. Popp proposed that the radiation might be both semi-periodic and coherent
Coherence (physics)
In physics, coherence is a property of waves that enables stationary interference. More generally, coherence describes all properties of the correlation between physical quantities of a wave....

. This hypothesis has not won general acceptance among scientists who have studied the evidence. Popp's group, however, constructed, tested, patented, and sought to market a device for measuring biophoton emissions as a means of assessing the ripeness and general food value of fruits and vegetables.

Russian, German, and other biophotonics experts, often adopting the term "biophotons" from Popp, have theorized, like Gurwitsch, that they may be involved in various cell functions, such as mitosis
Mitosis
Mitosis is the process by which a eukaryotic cell separates the chromosomes in its cell nucleus into two identical sets, in two separate nuclei. It is generally followed immediately by cytokinesis, which divides the nuclei, cytoplasm, organelles and cell membrane into two cells containing roughly...

, or even that they may be produced and detected by the DNA
DNA
Deoxyribonucleic acid is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms . The DNA segments that carry this genetic information are called genes, but other DNA sequences have structural purposes, or are involved in...

 in the cell nucleus
Cell nucleus
In cell biology, the nucleus is a membrane-enclosed organelle found in eukaryotic cells. It contains most of the cell's genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. The genes within these...

. In 1974 Dr. V.P.Kaznacheyev announced that his research team in Novosibirsk had detected intercellular communication by means of these rays. Until 1980s, Kaznacheyev and his team carried out about 12 000 experiments. Details of experiments are described in his book (in Russian).

Proponents additionally claim that studies have shown that injured cells will emit a higher biophoton rate than normal cells and that organisms with illnesses will likewise emit a brighter light
Light
Light or visible light is electromagnetic radiation that is visible to the human eye, and is responsible for the sense of sight. Visible light has wavelength in a range from about 380 nanometres to about 740 nm, with a frequency range of about 405 THz to 790 THz...

, which has been interpreted as implying a sort of distress signal. These ideas tend to support Gurwitsch's original idea that biophotons may be important for the development
Developmental biology
Developmental biology is the study of the process by which organisms grow and develop. Modern developmental biology studies the genetic control of cell growth, differentiation and "morphogenesis", which is the process that gives rise to tissues, organs and anatomy.- Related fields of study...

 of larger structures such as organ
Organ (anatomy)
In biology, an organ is a collection of tissues joined in structural unit to serve a common function. Usually there is a main tissue and sporadic tissues . The main tissue is the one that is unique for the specific organ. For example, main tissue in the heart is the myocardium, while sporadic are...

s and organism
Organism
In biology, an organism is any contiguous living system . In at least some form, all organisms are capable of response to stimuli, reproduction, growth and development, and maintenance of homoeostasis as a stable whole.An organism may either be unicellular or, as in the case of humans, comprise...

s.

However such conclusions are debatable. Injured cells are under higher amounts of oxidative stress
Oxidative stress
Oxidative stress represents an imbalance between the production and manifestation of reactive oxygen species and a biological system's ability to readily detoxify the reactive intermediates or to repair the resulting damage...

, which ultimately is the source of the light, and whether this constitutes a "distress signal" or simply a background chemical process is yet to be demonstrated. The difficulty of teasing out the effects of any supposed biophotons amid the other numerous chemical interactions between cells makes it difficult to devise a testable hypothesis. Most organisms are bathed in relatively high-intensity light that ought to swamp any signaling effect, although biophoton signaling might manifest through temporal patterns of distinct wavelengths or could mainly be used in deep tissues hidden from daylight (such as the human brain, which contains photoreceptor proteins
OPN3
Opsin-3 is a protein that, in humans, is encoded by the OPN3 gene. Alternative splicing of this gene results in multiple transcript variants encoding different protein isoforms.- Function :Opsins are members of the G protein-coupled receptor superfamily...

). There remains little evidence in the scientific literature to support the existence of such a signaling mechanism. Recent review article discusses various published theories on this kind of signaling and identifies around 30 experimental scientific articles in English in past 30 years which prove electromagnetic cellular interactions.

Direct illumination of the brain via the ear canal as a treatment for seasonal affective disorder
Seasonal affective disorder
Seasonal affective disorder , also known as winter depression, winter blues, summer depression, summer blues, or seasonal depression, is a mood disorder in which people who have normal mental health throughout most of the year experience depressive symptoms in the winter or summer, spring or autumn...

 is being researched by Valkee Ltd. and University of Oulu.

See also

  • Bioluminescence
    Bioluminescence
    Bioluminescence is the production and emission of light by a living organism. Its name is a hybrid word, originating from the Greek bios for "living" and the Latin lumen "light". Bioluminescence is a naturally occurring form of chemiluminescence where energy is released by a chemical reaction in...

  • Biophotonics
    Biophotonics
    The term biophotonics denotes a combination of biology and photonics, with photonics being the science and technology of generation, manipulation, and detection of photons, quantum units of light. Photonics is related to electronics in that it is believed that photons will play a similar central...

  • Biophysics
    Biophysics
    Biophysics is an interdisciplinary science that uses the methods of physical science to study biological systems. Studies included under the branches of biophysics span all levels of biological organization, from the molecular scale to whole organisms and ecosystems...

  • Chemiluminescence
  • Kirlian photography
    Kirlian photography
    Kirlian photography refers to a form of photogram made with voltage. It is named after Semyon Kirlian, who in 1939 accidentally discovered that if an object on a photographic plate is connected to a source of voltage an image is produced on the photographic plate.Kirlian's work, from 1939 onward,...

  • L-field
    L-field
    The L-field is a name proposed by the Yale Professor of Anatomy Harold Saxton Burr for the electromagnetic field of any organism. Burr held that the study of this field offered great promise for medicine since it exhibited measurable qualities that might be used in prognosis of disease, mood and...

  • Luminophore
    Luminophore
    A luminophore is an atom or atomic grouping in a chemical compound that manifests luminescence. There exist organic and inorganic luminophores. It should be stressed that the correct, textbook terminology is luminophore, not lumophore, although the latter term has been frequently but erroneously...

  • Phosphorescence
    Phosphorescence
    Phosphorescence is a specific type of photoluminescence related to fluorescence. Unlike fluorescence, a phosphorescent material does not immediately re-emit the radiation it absorbs. The slower time scales of the re-emission are associated with "forbidden" energy state transitions in quantum...

  • Prana
    Prana
    Prana is the Sanskrit word for "vital life" .It is one of the five organs of vitality or sensation, viz. prana "breath", vac "speech", chakshus "sight", shrotra "hearing", and manas "thought" Prana is the Sanskrit word for "vital life" (from the root "to fill", cognate to Latin plenus...

  • Vitalism
    Vitalism
    Vitalism, as defined by the Merriam-Webster dictionary, is#a doctrine that the functions of a living organism are due to a vital principle distinct from biochemical reactions...


Sources

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  • Cilento, G. Adam, W. From Free Radicals to Electronically Excited Species.: Free Radical Biology and Medicine. (1995) 19(1): 103-114.
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  • Katoaka, Y. Cui, Y.L. Yamagata, A. Niigaki, M. Hirohata, T. Oishi, N. Watanabe, Y.: Activity-Dependent Neural Tissue Oxidation Emits Intrinsic Ultraweak Photons. Biochemical and Biophysical Research Communications. 285 (2001): 1007-1011.
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  • Popp, F.A., Gurwitsch, A.A., Inaba, H., Slawinski, J., Cilento G., van Wijk, R., Chwirot B., and Nagl, W.: Biophoton Emission (Multi-Author Review), Experientia 44 (1988), 543-600.
  • Popp, F.A., Ruth, B., Bahr, W., Böhm, J., Grass, P., Grolig, G., Rattemeyer, M., Schmidt, H.G., and Wulle, P.:Emission of visible and ultraviolet radiation by active biological systems. Collective Phenomena (Gordon&Breach), Vol.3 (1981), pp. 187–214.
  • Popp, F.A., Yan, Yu: Delayed luminescence of biological systems in terms of coherent states. Physics Letters A 293 (2002), 93-97.
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  • Ursini, F. Barsacchi, R. Pelosi, G. Benassi, A.: Oxidative stress in the Rat Heart, Studies on Low-Level Chemiluminescence. Journal of Bioluminescence and Chemiluminescence. 4(1) (1989) 241-244.
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External links

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