Protein fingerprinting
Encyclopedia
Protein fingerprinting can refer to any of the several methods for identifying or differentiating proteins:
- Peptide mass fingerprintingPeptide mass fingerprintingPeptide mass fingerprinting is an analytical technique for protein identification that was developed in 1993 by several groups independently. In this method, the unknown protein of interest is first cleaved into smaller peptides, whose absolute masses can be accurately measured with a mass...
, a method developed in 1993 that uses protein mass spectrometry following SDS-PAGE - Older techniques using two-dimensional chromatographyChromatographyChromatography is the collective term for a set of laboratory techniques for the separation of mixtures....
and/or protein electrophoresisProtein electrophoresisProtein electrophoresis is a method for analysing the proteins in a fluid or an extract. The electrophoresis may be performed with a small volume of sample in a number of alternative ways with or without a supporting medium: SDS polyacrylamide gel electrophoresis Protein electrophoresis is a method...