Up-and-down procedure
Encyclopedia
Up-and-down procedure is an alternative to the acute toxicity
Toxicity
Toxicity is the degree to which a substance can damage a living or non-living organisms. Toxicity can refer to the effect on a whole organism, such as an animal, bacterium, or plant, as well as the effect on a substructure of the organism, such as a cell or an organ , such as the liver...

test.

It requires fewer animals to achieve similar accuracy as the LD50 test. However, the U.S. Food and Drug Administration has begun to approve non-animal alternatives, in response to research cruelty concerns and the lack of validity/sensitivity of animal tests as they relate to humans.
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