I am studying the mecanism of vascular smooth muscle relaxation by cyclic nucleotides (CNs) in rat aorta (organ bath) and A7r5 cells (patch clamp).

So far, my results are:

- Calcium channels L-type were not inibited by CNs (using conventional pacth- clamp)
- Potassium channels were not activated by CNs (using conventional pacth- clamp)

I saw several papers mentioning inibition of Calcium channels L-type and other papers mentioning activation of Potassium channels (Kv or BKca).

I did not observed that results.

But I obtained good results with some inhibitors (nifedipine, 4-AP) and activators (Bay K, FLP) discribed in the literature but nothing with NCs (neither inhibiton nor activation)and the curves of calcium currents and potassium currents were very good (good seal also).


Any ideas on how to get that results?