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ACES (buffer)
Encyclopedia
ACES is the common abbreviation for the compound N-(2-Acetamido)-2-aminoethanesulfonic acid.
ACES is one of Good's buffers
developed in the 1960s to provide buffers with pH
ranging from 6.15-8.35 for use in various applications. With a pKa
of 6.9, it is often used as a buffering agent
in biological
and biochemical
research. It is a zwitterion
ic buffer with a useful buffering range of 6.1-7.5. The pioneering publication by Good and his co-workers described the synthesis and physical properties of ACES buffer.
ACES use in isoelectric focusing
of proteins has also been documented. Use of ACES has been published in a protocol for the analysis of bacterial autolysins in a discontinuous SDS-PAGE
system. Potential inhibition of ACES and other Good buffers has been investigated in γ-aminobutyric acid receptor binding to rat brain synaptic membranes.
ACES is one of Good's buffers
Good's buffers
Good's buffers are twelve buffering agents selected and described by Norman Good and colleagues in 1966. Good selected the buffers based on a number of criteria which make them candidates for use in biochemistry and biological research...
developed in the 1960s to provide buffers with pH
PH
In chemistry, pH is a measure of the acidity or basicity of an aqueous solution. Pure water is said to be neutral, with a pH close to 7.0 at . Solutions with a pH less than 7 are said to be acidic and solutions with a pH greater than 7 are basic or alkaline...
ranging from 6.15-8.35 for use in various applications. With a pKa
PKA
PKA, pKa, or other similar variations may stand for:* pKa, the symbol for the acid dissociation constant at logarithmic scale* Protein kinase A, a class of cAMP-dependent enzymes* Pi Kappa Alpha, the North-American social fraternity...
of 6.9, it is often used as a buffering agent
Buffering agent
A buffering agent is a weak acid or base used to maintain the acidity of a solution at a chosen value. The function of a buffering agent is to prevent a rapid change in pH when acids or bases are added to the solution. Buffering agents have variable properties—some are more soluble than others;...
in biological
Biology
Biology is a natural science concerned with the study of life and living organisms, including their structure, function, growth, origin, evolution, distribution, and taxonomy. Biology is a vast subject containing many subdivisions, topics, and disciplines...
and biochemical
Biochemistry
Biochemistry, sometimes called biological chemistry, is the study of chemical processes in living organisms, including, but not limited to, living matter. Biochemistry governs all living organisms and living processes...
research. It is a zwitterion
Zwitterion
In chemistry, a zwitterion is a neutral molecule with a positive and a negative electrical charge at different locations within that molecule. Zwitterions are sometimes also called inner salts.-Examples:...
ic buffer with a useful buffering range of 6.1-7.5. The pioneering publication by Good and his co-workers described the synthesis and physical properties of ACES buffer.
Applications
ACES had been used to develop buffers for both agarose and polyacrylamide gel electrophoresis.ACES use in isoelectric focusing
Isoelectric focusing
Isoelectric focusing , also known as electrofocusing, is a technique for separating different molecules by their electric charge differences...
of proteins has also been documented. Use of ACES has been published in a protocol for the analysis of bacterial autolysins in a discontinuous SDS-PAGE
SDS-PAGE
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a collection of related techniques widely used in biochemistry, forensics, genetics and molecular biology to separate proteins according to their electrophoretic mobility...
system. Potential inhibition of ACES and other Good buffers has been investigated in γ-aminobutyric acid receptor binding to rat brain synaptic membranes.