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The Barossa Valley railway line, is a freight only line, running from Gawler Central Station to the Penrice Soda Works in the Barossa Valley, 84 km north of Adelaide.

Traffic is light, only the daily Genesee and Wyoming Australia

Genesee and Wyoming Australia

Genesee and Wyoming Australia is an Australian rail freight operator based in Adelaide, South Australia. It is a 100 percent owned subsidiary of Genesee and Wyoming Inc based in the USA, and was formed in June 2006 to run the South Australian operations of the Australian Railroad Group joint...

 operated 'Stonie'

Penrice Stone Train

The Penrice Stone Train is a freight train in South Australia that operates from Penrice in the Barossa Valley to Osborne in Adelaide's north-western suburbs...

 uses the line to ferry soda products from Penrice to Osborne

Osborne, South Australia

Osborne is a north-western suburb of Adelaide, South Australia, Australia 19 km from the CBD, in the City of Port Adelaide Enfield. It is on the LeFevre Peninsula, adjacent to Outer Harbor, Taperoo and North Haven...

.

Regular passenger services have not been seen on the line since 2003, with the withdrawal of the Barossa Wine Train. Commuter passenger services were earlier withdrawn on 16 December 1968.

In 2010, the track between Angaston and Nuriootpa was ripped up and a bike path was put in place.