Burst kinetics
Encyclopedia
Burst kinetics is a form of enzyme kinetics
that refers to an initial high velocity of enzymatic turnover when adding enzyme to substrate. This initial period of high velocity product formation is referred to as the "Burst Phase". This period is observed as the enzymes become saturated with substrate up until all enzymes are saturated. Once all enzymes are saturated, the Burst Phase gives way to a linear reaction velocity. An example of a burst kinetics is observed in Step 6 of Glycolysis
involving GAP Dehydrogenase (GAPDH) and the reduction of NAD+ to NADH. Furthermore, the production of NADH can be measured as it is yellow and absorbs at 340 nm.
Enzyme kinetics
Enzyme kinetics is the study of the chemical reactions that are catalysed by enzymes. In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction investigated...
that refers to an initial high velocity of enzymatic turnover when adding enzyme to substrate. This initial period of high velocity product formation is referred to as the "Burst Phase". This period is observed as the enzymes become saturated with substrate up until all enzymes are saturated. Once all enzymes are saturated, the Burst Phase gives way to a linear reaction velocity. An example of a burst kinetics is observed in Step 6 of Glycolysis
Glycolysis
Glycolysis is the metabolic pathway that converts glucose C6H12O6, into pyruvate, CH3COCOO− + H+...
involving GAP Dehydrogenase (GAPDH) and the reduction of NAD+ to NADH. Furthermore, the production of NADH can be measured as it is yellow and absorbs at 340 nm.