C7.GAT protein
Encyclopedia
The C7.GAT protein is a zinc finger protein
based on the C7 protein
(itself based on the murine Zif268
). It features an alternative zinc finger 3 alpha helix
sequence, preventing the target site overlap caused by the aspartic acid
residue of the finger 3 of C7. The sequence of this third finger is TSG-N-LVR according to the single letter amino acid code. As the name suggest, the target site of finger 3 is altered to 5'-GAT-3', giving the overall protein a target of 5'-GCGTGGGAT-3'.
C7.GAT is used in studies investigating the effects of altering zinc finger
alpha helix sequence on the target of the altered zinc finger, as well as the affinity and specificty of these proteins to their targets.
Zinc finger protein
A zinc finger protein is a DNA-binding protein domain consisting of zinc fingers ranging from two in the Drosophila regulator ADR1, the more common three in mammalian Sp1 up to nine in TFIIIA...
based on the C7 protein
C7 protein
C7 protein is an engineered zinc finger protein based on the murine ZFP, Zif268 and discovered by Wu et al. in 1994 . It shares the same zinc finger 2 and zinc finger 3 of Zif268, but differs in the sequence of finger 1...
(itself based on the murine Zif268
Zif268
EGR-1 also known as Zif268 or NGFI-A is a protein that in humans is encoded by the EGR1 gene....
). It features an alternative zinc finger 3 alpha helix
Alpha helix
A common motif in the secondary structure of proteins, the alpha helix is a right-handed coiled or spiral conformation, in which every backbone N-H group donates a hydrogen bond to the backbone C=O group of the amino acid four residues earlier...
sequence, preventing the target site overlap caused by the aspartic acid
Aspartic acid
Aspartic acid is an α-amino acid with the chemical formula HOOCCHCH2COOH. The carboxylate anion, salt, or ester of aspartic acid is known as aspartate. The L-isomer of aspartate is one of the 20 proteinogenic amino acids, i.e., the building blocks of proteins...
residue of the finger 3 of C7. The sequence of this third finger is TSG-N-LVR according to the single letter amino acid code. As the name suggest, the target site of finger 3 is altered to 5'-GAT-3', giving the overall protein a target of 5'-GCGTGGGAT-3'.
C7.GAT is used in studies investigating the effects of altering zinc finger
Zinc finger
Zinc fingers are small protein structural motifs that can coordinate one or more zinc ions to help stabilize their folds. They can be classified into several different structural families and typically function as interaction modules that bind DNA, RNA, proteins, or small molecules...
alpha helix sequence on the target of the altered zinc finger, as well as the affinity and specificty of these proteins to their targets.