ChiRP-Seq
Encyclopedia
ChIRP-Seq is a high-throughput sequencing method to discover RNA-bound DNA and proteins. The RNA sequences of interest are hybridized to oligonucleotide tiles using biotin
/streptavidin
. The chromatin fraction that is bound to the beads is then determined using high-throughput sequencing.
Biotin
Biotin, also known as Vitamin H or Coenzyme R, is a water-soluble B-complex vitamin discovered by Bateman in 1916. It is composed of a ureido ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring...
/streptavidin
Streptavidin
Streptavidin is a 60000 dalton protein purified from the bacterium Streptomyces avidinii. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin . With a dissociation constant on the order of ≈10-14 mol/L, the binding of biotin to streptavidin is one of the strongest...
. The chromatin fraction that is bound to the beads is then determined using high-throughput sequencing.