Chu 13
Encyclopedia
CHU 13 medium is a culture medium used in microbiology
for the growth of certain algal species, first published by S.P. Chu in 1942. It is used as growth medium for the biofuel
candidate alga Botryococcus braunii
.
CHU 13 includes essential minerals and trace elements
that are required by algae for growth, but does not include a carbon source and so is only appropriate for growth of phototroph
s. It can be prepared as either a liquid medium or as an agar
medium.
The remaining volume is pure, de-ionized water.
Because it is difficult to weigh out some of the trace minerals, it is advisable to create a mixture of all components at a large concentration, such as a thousand times these measures, and then mix with the appropriate amount of (pure, de-ionized) water. Correct pH to 7.5, and then autoclave.
Microbiology
Microbiology is the study of microorganisms, which are defined as any microscopic organism that comprises either a single cell , cell clusters or no cell at all . This includes eukaryotes, such as fungi and protists, and prokaryotes...
for the growth of certain algal species, first published by S.P. Chu in 1942. It is used as growth medium for the biofuel
Biofuel
Biofuel is a type of fuel whose energy is derived from biological carbon fixation. Biofuels include fuels derived from biomass conversion, as well as solid biomass, liquid fuels and various biogases...
candidate alga Botryococcus braunii
Botryococcus braunii
Botryococcus braunii is a green, pyramid shaped planktonic microalga that is of potentially great importance in the field of biotechnology. Colonies held together by a lipid biofilm matrix can be found in temperate or tropical oligotrophic lakes and estuaries, and will bloom when in the presence...
.
CHU 13 includes essential minerals and trace elements
Chemical element
A chemical element is a pure chemical substance consisting of one type of atom distinguished by its atomic number, which is the number of protons in its nucleus. Familiar examples of elements include carbon, oxygen, aluminum, iron, copper, gold, mercury, and lead.As of November 2011, 118 elements...
that are required by algae for growth, but does not include a carbon source and so is only appropriate for growth of phototroph
Phototroph
Phototrophs are the organisms that carry out photosynthesis to acquire energy. They use the energy from sunlight to convert carbon dioxide and water into organic material to be utilized in cellular functions such as biosynthesis and respiration.Most phototrophs are autotrophs, also known as...
s. It can be prepared as either a liquid medium or as an agar
Agar
Agar or agar-agar is a gelatinous substance derived from a polysaccharide that accumulates in the cell walls of agarophyte red algae. Throughout history into modern times, agar has been chiefly used as an ingredient in desserts throughout Asia and also as a solid substrate to contain culture medium...
medium.
| Compound | mg/L |
|---|---|
| KNO3 Potassium nitrate Potassium nitrate is a chemical compound with the formula KNO3. It is an ionic salt of potassium ions K+ and nitrate ions NO3−.It occurs as a mineral niter and is a natural solid source of nitrogen. Its common names include saltpetre , from medieval Latin sal petræ: "stone salt" or possibly "Salt... |
400 |
| K2HPO4 | 80 |
| CaCl2 Calcium chloride Calcium chloride, CaCl2, is a salt of calcium and chlorine. It behaves as a typical ionic halide, and is solid at room temperature. Common applications include brine for refrigeration plants, ice and dust control on roads, and desiccation... dihydrate |
107 |
| MgSO4 Magnesium sulfate Magnesium sulfate is a chemical compound containing magnesium, sulfur and oxygen, with the formula MgSO4. It is often encountered as the heptahydrate epsomite , commonly called Epsom salt, from the town of Epsom in Surrey, England, where the salt was distilled from the springs that arise where the... heptahydrate |
200 |
| Ferric Citrate | 20 |
| Citric acid Citric acid Citric acid is a weak organic acid. It is a natural preservative/conservative and is also used to add an acidic, or sour, taste to foods and soft drinks... |
100 |
| CoCl2 | 0.02 |
| H3BO3 Boric acid Boric acid, also called hydrogen borate or boracic acid or orthoboric acid or acidum boricum, is a weak acid of boron often used as an antiseptic, insecticide, flame retardant, as a neutron absorber, and as a precursor of other chemical compounds. It exists in the form of colorless crystals or a... |
5.72 |
| MnCl2 tetrahydrate | 3.62 |
| ZnSO4 heptahydrate | 0.44 |
| CuSO4 pentahydrate | 0.16 |
| Na2MoO4 | 0.084 |
| 0.072 N H2SO4 Sulfuric acid Sulfuric acid is a strong mineral acid with the molecular formula . Its historical name is oil of vitriol. Pure sulfuric acid is a highly corrosive, colorless, viscous liquid. The salts of sulfuric acid are called sulfates... |
1 drop |
The remaining volume is pure, de-ionized water.
Because it is difficult to weigh out some of the trace minerals, it is advisable to create a mixture of all components at a large concentration, such as a thousand times these measures, and then mix with the appropriate amount of (pure, de-ionized) water. Correct pH to 7.5, and then autoclave.
External links
- http://answers.yahoo.com/question/index?qid=20081217192542AAkM62U (A recipe for 2X CHU 13 media)