Degradome sequencing
Encyclopedia
Degradome sequencing, also referred to as parallel analysis of RNA ends (PARE
), is a modified 5'-rapid amplification of cDNA ends (RACE) with high-throughput deep sequencing (SBS) method. Degradome sequencing provides a comprehensive means of analyzing patterns of RNA degradation.
Degradome sequencing has been use to identify microRNA (miRNA
) cleavage sites, because miRNAs can cause endonucleolytic cleavage of mRNA by extensive and often perfect complementarity to mRNAs. Degradome sequencing revealed many known and novel plant miRNA
(siRNA
) targets. Recently, degradome sequencing also has been applied to identify animal (human and mouse) miRNA-derived cleavages.
Pare
The Pare people are members of an ethnic group indigenous to the Pare Mountains of northern Tanzania, which are part of the Kilimanjaro Region of Tanzania. Pareland is also known as Vuasu...
), is a modified 5'-rapid amplification of cDNA ends (RACE) with high-throughput deep sequencing (SBS) method. Degradome sequencing provides a comprehensive means of analyzing patterns of RNA degradation.
Degradome sequencing has been use to identify microRNA (miRNA
Mirna
Mirna may refer to:geographical entities* Mirna , a river in Istria, Croatia* Mirna , a river in Slovenia, tributary of the river Sava* Mirna , a settlement in the municipality of Mirna in Southeastern Sloveniapeople...
) cleavage sites, because miRNAs can cause endonucleolytic cleavage of mRNA by extensive and often perfect complementarity to mRNAs. Degradome sequencing revealed many known and novel plant miRNA
Mirna
Mirna may refer to:geographical entities* Mirna , a river in Istria, Croatia* Mirna , a river in Slovenia, tributary of the river Sava* Mirna , a settlement in the municipality of Mirna in Southeastern Sloveniapeople...
(siRNA
Sírna
Sírna Sáeglach , son of Dian mac Demal, son of Demal mac Rothechtaid, son of Rothechtaid mac Main, was, according to medieval Irish legend and historical tradition, a High King of Ireland...
) targets. Recently, degradome sequencing also has been applied to identify animal (human and mouse) miRNA-derived cleavages.
External links
- starBase database: a database for exploring mciroRNA cleavage sites from degradome sequencingDegradome sequencingDegradome sequencing , also referred to as parallel analysis of RNA ends , is a modified 5'-rapid amplification of cDNA ends with high-throughput deep sequencing method...
(Degradome-Seq) data.