Dichlorphenolindophenol
Overview
Chemical compound
A chemical compound is a pure chemical substance consisting of two or more different chemical elements that can be separated into simpler substances by chemical reactions. Chemical compounds have a unique and defined chemical structure; they consist of a fixed ratio of atoms that are held together...
used as a redox dye
Redox indicator
A redox indicator is an indicator that undergoes a definite color change at a specific electrode potential....
. Oxidized, DCPIP is blue; reduced
Redox
Redox reactions describe all chemical reactions in which atoms have their oxidation state changed....
, DCPIP is colorless.
]] with DCPIP. Due to the reductions that occur during the light reactions, DCPIP can be substituted for NADPH, the final electron carrier in the light reactions. The light reactions will reduce the DCPIP and turn it colorless. As DCPIP is reduced and becomes colorless, the following increase in light transmittance can be measured by a spectrophotometer.
DCPIP is a redox dye
Redox indicator
A redox indicator is an indicator that undergoes a definite color change at a specific electrode potential....
commonly used as a monitor of the light reactions in photosynthesis
Photosynthesis
Photosynthesis is a chemical process that converts carbon dioxide into organic compounds, especially sugars, using the energy from sunlight. Photosynthesis occurs in plants, algae, and many species of bacteria, but not in archaea. Photosynthetic organisms are called photoautotrophs, since they can...
because it is an electron
Electron
The electron is a subatomic particle with a negative elementary electric charge. It has no known components or substructure; in other words, it is generally thought to be an elementary particle. An electron has a mass that is approximately 1/1836 that of the proton...
acceptor that is blue when oxidized and colorless when reduced
Redox
Redox reactions describe all chemical reactions in which atoms have their oxidation state changed....
.
Unanswered Questions
