Excitation filter
Encyclopedia
An excitation filter is a high quality optical-glass filter commonly used in fluorescence microscopy
and spectroscopic
applications for selection of the excitation wavelength of light from a light source. Most excitation filters select light of relatively short wavelengths from an excitation light source (as light with shorter wavelengths have higher energy, according to the Planck equation
). The excitation filters used may come in 2 main types - short pass filters and band pass filters. Variations of these filters exist in the form of notch filters or deep blocking filters (commonly employed as emission filters). Other forms of excitation filters include the use of monochromators, wedge prisms coupled with a narrow slit (for selection of the excitation light) and the use of holographic diffraction gratings, etc [for beam diffraction of white laser light into the required excitation wavelength (selected for by a narrow slit)].
Fluorescence microscope
A fluorescence microscope is an optical microscope used to study properties of organic or inorganic substances using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption...
and spectroscopic
Spectroscopy
Spectroscopy is the study of the interaction between matter and radiated energy. Historically, spectroscopy originated through the study of visible light dispersed according to its wavelength, e.g., by a prism. Later the concept was expanded greatly to comprise any interaction with radiative...
applications for selection of the excitation wavelength of light from a light source. Most excitation filters select light of relatively short wavelengths from an excitation light source (as light with shorter wavelengths have higher energy, according to the Planck equation
). The excitation filters used may come in 2 main types - short pass filters and band pass filters. Variations of these filters exist in the form of notch filters or deep blocking filters (commonly employed as emission filters). Other forms of excitation filters include the use of monochromators, wedge prisms coupled with a narrow slit (for selection of the excitation light) and the use of holographic diffraction gratings, etc [for beam diffraction of white laser light into the required excitation wavelength (selected for by a narrow slit)].