HPTLC
Encyclopedia
High performance thin layer chromatography (HPTLC) is an enhanced form of thin layer chromatography
(TLC). A number of enhancements can be made to the basic method of thin layer chromatography to automate the different steps, to increase the resolution achieved and to allow more accurate quantitative measurements.
Automation is useful to overcome the uncertainty in droplet size and position when the sample is applied to the TLC plate by hand. One recent approach to automation has been the use of piezoelectric devices and inkjet printers for applying the sample.
The spot capacity (analogous to peak capacity in HPLC
) can be increased by developing the plate with two different solvents. After the plate is exposed to the first solvent, the solvent is removed, the plate is rotated 90° and developed with a second solvent. If the two solvents show different selectivity, then the spots may be spread over the entire surface of the plate. This is obviously a form of two-dimensional chromatography
.
Thin layer chromatography
Thin layer chromatography is a chromatography technique used to separate mixtures. Thin layer chromatography is performed on a sheet of glass, plastic, or aluminum foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose...
(TLC). A number of enhancements can be made to the basic method of thin layer chromatography to automate the different steps, to increase the resolution achieved and to allow more accurate quantitative measurements.
Automation is useful to overcome the uncertainty in droplet size and position when the sample is applied to the TLC plate by hand. One recent approach to automation has been the use of piezoelectric devices and inkjet printers for applying the sample.
The spot capacity (analogous to peak capacity in HPLC
High-performance liquid chromatography
High-performance liquid chromatography , HPLC, is a chromatographic technique that can separate a mixture of compounds and is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of the mixture.HPLC typically utilizes different types of stationary...
) can be increased by developing the plate with two different solvents. After the plate is exposed to the first solvent, the solvent is removed, the plate is rotated 90° and developed with a second solvent. If the two solvents show different selectivity, then the spots may be spread over the entire surface of the plate. This is obviously a form of two-dimensional chromatography
Two-dimensional chromatography
Two-dimensional chromatography is a type of chromatographic technique in which the injected sample is separated by passing through two different separation stages. This is done by injecting the eluent from the first column onto a second column...
.