Histochemical tracer
Encyclopedia
A histochemical tracer is a compound used to reveal the location of cell
s and track neuronal
projections. A neuronal tracer may be retrograde, anterograde, or work in both directions. A retrograde tracer is taken up in the terminal of the neuron and transported to the cell body, whereas an anterograde tracer moves away from the cell body of the neuron.
Cell (biology)
The cell is the basic structural and functional unit of all known living organisms. It is the smallest unit of life that is classified as a living thing, and is often called the building block of life. The Alberts text discusses how the "cellular building blocks" move to shape developing embryos....
s and track neuronal
Neuron
A neuron is an electrically excitable cell that processes and transmits information by electrical and chemical signaling. Chemical signaling occurs via synapses, specialized connections with other cells. Neurons connect to each other to form networks. Neurons are the core components of the nervous...
projections. A neuronal tracer may be retrograde, anterograde, or work in both directions. A retrograde tracer is taken up in the terminal of the neuron and transported to the cell body, whereas an anterograde tracer moves away from the cell body of the neuron.
List
- Diamidino yellow
- Fast blue
- Horseradish peroxidaseHorseradish peroxidaseThe enzyme horseradish peroxidase , found in horseradish, is used extensively in biochemistry applications primarily for its ability to amplify a weak signal and increase detectability of a target molecule.-Applications:...
- retrograde - Cholera toxin BCholera toxinCholera toxin is a protein complex secreted by the bacterium Vibrio cholerae. CTX is responsible for the massive, watery diarrhea characteristic of cholera infection.- Structure :...
- Pseudorabies virusPseudorabiesPseudorabies is a viral disease in swine that is endemic in most parts of the world. It is caused by Suid herpesvirus 1 , which is also called Pseudorabies virus and is also known as Aujeszky's disease, and in cattle as mad itch. PRV is considered to be the most economically important viral...
- HydroxystilbamidineHydroxystilbamidineHydroxystilbamidine is a fluorescent dye that emits different frequencies of light when bound to DNA and RNA. It is used as a retrograde tracer for outlining neurons, and as a histochemical stain....
- retrograde - Texas RedTexas RedTexas Red or sulforhodamine 101 acid chloride is a red fluorescent dye, used in histology for staining cell specimens, for sorting cells with fluorescent-activated cell sorting machines, in fluorescence microscopy applications, and in immunohistochemistry.Texas Red fluoresces at about 615 nm,...
- Fluorescein isothiocyanateFluorescein isothiocyanateFluorescein isothiocyanate is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group , replacing a hydrogen atom on the bottom ring of the structure...