Huygens Software
Encyclopedia
Huygens software refers to different multiplatform microscope image processing
packages from Scientific Volume Imaging, made for restoring 2D and 3D
microscopy
images or time series
and analyzing and visualizing them.
The restoration is based on different deconvolution
algorithm
s, that permit the recovery of objects from images that are degraded by blurring and noise. In microscopy
the blurring is largely due to diffraction
limited imaging by the instrument; the noise is usually photon noise.
The scientific visualization
of 3D volume data is based on the simulated fluorescence process algorithm
(SFP), but isosurface
s and maximum intensity projection
s are also used for object analysis and colocalization
.
Huygens software is named after the Dutch physicist Christiaan Huygens who is perhaps best known for his argument that light behaves like waves. Since
wave diffraction plays a key role in the Huygens Software, it was named after him.
Microscope image processing
Microscope image processing is a broad term that covers the use of digital image processing techniques to process, analyze and present images obtained from a microscope. Such processing is now commonplace in a number of diverse fields such as medicine, biological research, cancer research, drug...
packages from Scientific Volume Imaging, made for restoring 2D and 3D
Three-dimensional space
Three-dimensional space is a geometric 3-parameters model of the physical universe in which we live. These three dimensions are commonly called length, width, and depth , although any three directions can be chosen, provided that they do not lie in the same plane.In physics and mathematics, a...
microscopy
Microscopy
Microscopy is the technical field of using microscopes to view samples and objects that cannot be seen with the unaided eye...
images or time series
Time series
In statistics, signal processing, econometrics and mathematical finance, a time series is a sequence of data points, measured typically at successive times spaced at uniform time intervals. Examples of time series are the daily closing value of the Dow Jones index or the annual flow volume of the...
and analyzing and visualizing them.
The restoration is based on different deconvolution
Deconvolution
In mathematics, deconvolution is an algorithm-based process used to reverse the effects of convolution on recorded data. The concept of deconvolution is widely used in the techniques of signal processing and image processing...
algorithm
Algorithm
In mathematics and computer science, an algorithm is an effective method expressed as a finite list of well-defined instructions for calculating a function. Algorithms are used for calculation, data processing, and automated reasoning...
s, that permit the recovery of objects from images that are degraded by blurring and noise. In microscopy
Microscopy
Microscopy is the technical field of using microscopes to view samples and objects that cannot be seen with the unaided eye...
the blurring is largely due to diffraction
Diffraction
Diffraction refers to various phenomena which occur when a wave encounters an obstacle. Italian scientist Francesco Maria Grimaldi coined the word "diffraction" and was the first to record accurate observations of the phenomenon in 1665...
limited imaging by the instrument; the noise is usually photon noise.
The scientific visualization
Scientific visualization
Scientific visualization is an interdisciplinary branch of science according to Friendly "primarily concerned with the visualization of three-dimensional phenomena , where the emphasis is on realistic renderings of volumes, surfaces, illumination sources, and so forth, perhaps...
of 3D volume data is based on the simulated fluorescence process algorithm
Simulated fluorescence process algorithm
The Simulated Fluorescence Process is a computing algorithm used for scientific visualization of 3D data from, for example, fluorescence microscopes...
(SFP), but isosurface
Isosurface
An isosurface is a three-dimensional analog of an isoline. It is a surface that represents points of a constant value within a volume of space; in other words, it is a level set of a continuous function whose domain is 3D-space.Isosurfaces are normally displayed using computer graphics, and are...
s and maximum intensity projection
Maximum intensity projection
In scientific visualization, a maximum intensity projection is a volume rendering method for 3D data that projects in the visualization plane the voxels with maximum intensity that fall in the way of parallel rays traced from the viewpoint to the plane of projection...
s are also used for object analysis and colocalization
Colocalization
In fluorescence microscopy, colocalization refers to observation of the spatial overlap between two different fluorescent labels, each having a separate emission wavelength, to see if the different "targets" are located in the same area of the cell or very near to one another...
.
Huygens software is named after the Dutch physicist Christiaan Huygens who is perhaps best known for his argument that light behaves like waves. Since
wave diffraction plays a key role in the Huygens Software, it was named after him.