Lys-N
Encyclopedia
Lys-N is a metalloendopeptidase
found in the Japanese mushroom Grifola frondosa
that cleaves proteins on the amino side of lysine
residues.
Lys-N is becoming a popular protease used for protein digestion in proteomics
experiments. The combination Lys-N proteolytic peptides and mass spectrometry sequencing with ETD creates tandem mass spectra
composed mostly of amino terminal peptide fragment ions.
This fragmentation pattern facilitates the applicability of these spectra for de novo proteomics.
Metalloendopeptidase
A metalloendopeptidase is an enzyme that functions as a metalloproteinase endopeptidase....
found in the Japanese mushroom Grifola frondosa
Grifola frondosa
Grifola frondosa is a polypore mushroom that grows in clusters at the base of trees, particularly oaks. The mushroom is commonly known among English speakers as Hen-of-the-Woods, Ram's Head and Sheep's Head...
that cleaves proteins on the amino side of lysine
Lysine
Lysine is an α-amino acid with the chemical formula HO2CCH4NH2. It is an essential amino acid, which means that the human body cannot synthesize it. Its codons are AAA and AAG....
residues.
Lys-N is becoming a popular protease used for protein digestion in proteomics
Proteomics
Proteomics is the large-scale study of proteins, particularly their structures and functions. Proteins are vital parts of living organisms, as they are the main components of the physiological metabolic pathways of cells. The term "proteomics" was first coined in 1997 to make an analogy with...
experiments. The combination Lys-N proteolytic peptides and mass spectrometry sequencing with ETD creates tandem mass spectra
Tandem mass spectrometry
Tandem mass spectrometry, also known as MS/MS or MS2, involves multiple steps of mass spectrometry selection, with some form of fragmentation occurring in between the stages.-Tandem MS instruments:...
composed mostly of amino terminal peptide fragment ions.
This fragmentation pattern facilitates the applicability of these spectra for de novo proteomics.
See also
- Mass spectrometryMass spectrometryMass spectrometry is an analytical technique that measures the mass-to-charge ratio of charged particles.It is used for determining masses of particles, for determining the elemental composition of a sample or molecule, and for elucidating the chemical structures of molecules, such as peptides and...
- ProteomicsProteomicsProteomics is the large-scale study of proteins, particularly their structures and functions. Proteins are vital parts of living organisms, as they are the main components of the physiological metabolic pathways of cells. The term "proteomics" was first coined in 1997 to make an analogy with...
- Tandem mass spectrometryTandem mass spectrometryTandem mass spectrometry, also known as MS/MS or MS2, involves multiple steps of mass spectrometry selection, with some form of fragmentation occurring in between the stages.-Tandem MS instruments:...
- Bottom-up proteomicsBottom-up proteomicsBottom-up proteomics is a common method to identify proteins and characterize their amino acid sequences and post-translational modifications by proteolytic digestion of proteins prior to analysis by mass spectrometry. The proteins may first be purified by a method such as gel electrophoresis...
External links
- The MEROPSMeropsMerops may refer to:* Merops , a genus of bee-eaters.* MEROPS, an on-line database for peptidases.It may also refer to several figures from Greek mythology:* King of Ethiopia, husband of Clymene, who lay with Helios and bore Phaethon...
online database for peptidases and their inhibitors: M35.004 - Coverage on Genomeweb
- Enzyme entry at SWISS-PROT