Machine perfusion
Encyclopedia
Machine perfusion is a technique used in organ transplantation as a means of preserving the organ
s which are to be transplanted. To some degree, it emulates natural perfusion
. So far it has mainly been used in kidney transplantation
. It is an alternative to cold storage
(CS). Its clinical and cost-effectiveness are still subject to research.
Hypothermic perfusion has given the longest storage time for canine kidneys with the best result being 8 day storage. This experimental model used a storage temperature of 8°C and a Plasma Protein Fraction (PPF) based perfusate. The octanoic acid content of PPF was found to influence the results of preservation in five-day storage. Both octanoic acid and oleic acid
stimulated oxygen consumption to a similar degree during hypothermic perfusion suggesting that the detrimental effect of Octanoic acid was due to direct metabolic stimulation rather than uncoupling of oxidatative phosphorylation.
Organ (anatomy)
In biology, an organ is a collection of tissues joined in structural unit to serve a common function. Usually there is a main tissue and sporadic tissues . The main tissue is the one that is unique for the specific organ. For example, main tissue in the heart is the myocardium, while sporadic are...
s which are to be transplanted. To some degree, it emulates natural perfusion
Perfusion
In physiology, perfusion is the process of nutritive delivery of arterial blood to a capillary bed in the biological tissue. The word is derived from the French verb "perfuser" meaning to "pour over or through."...
. So far it has mainly been used in kidney transplantation
Kidney transplantation
Kidney transplantation or renal transplantation is the organ transplant of a kidney into a patient with end-stage renal disease. Kidney transplantation is typically classified as deceased-donor or living-donor transplantation depending on the source of the donor organ...
. It is an alternative to cold storage
Cold storage
Cold storage may refer to:* A form of refrigerated storage* Cold Storage, a musician also known as Tim Wright * Cold Storage , a supermarket found in Singapore and Malaysia* COLD...
(CS). Its clinical and cost-effectiveness are still subject to research.
Hypothermic perfusion has given the longest storage time for canine kidneys with the best result being 8 day storage. This experimental model used a storage temperature of 8°C and a Plasma Protein Fraction (PPF) based perfusate. The octanoic acid content of PPF was found to influence the results of preservation in five-day storage. Both octanoic acid and oleic acid
Oleic acid
Oleic acid is a monounsaturated omega-9 fatty acid found in various animal and vegetable fats. It has the formula CH37CH=CH7COOH. It is an odorless, colourless oil, although commercial samples may be yellowish. The trans isomer of oleic acid is called elaidic acid...
stimulated oxygen consumption to a similar degree during hypothermic perfusion suggesting that the detrimental effect of Octanoic acid was due to direct metabolic stimulation rather than uncoupling of oxidatative phosphorylation.