Murtoa is no longer used as a passenger station following the withdrawal of the V/Line Dimboola service in 1993. The station however still functions as an important location for Freight Australia where it serves as the junction for the Hopetoun branch.

The station used to have a dock platform for the Hopetoun branch. People in Murtoa have called on The Overland, the interstate rail journey that runs from Adelaide

Adelaide

Adelaide is the capital city of South Australia and the fifth-largest city in Australia. Adelaide has an estimated population of more than 1.2 million...

 to Melbourne

Melbourne

Melbourne is the capital and most populous city in the state of Victoria, and the second most populous city in Australia. The Melbourne City Centre is the hub of the greater metropolitan area and the Census statistical division—of which "Melbourne" is the common name. As of June 2009, the greater...

 to make Murtoa an additional stop for the train service.