Naked DNA
Encyclopedia
Naked DNA is histone
-free DNA
that is passed from cell to cell during a gene transfer process called transformation
or transfection
. In transformation , purified or naked DNA is taken up by the recipient cell which will give the recipient cell a new characteristic or phenotype
. Transfection differs from transformation since the DNA is not generally incorporated into the cell's genome, it is only transiently expressed.
In the field of DNA vaccines or genetic immunization, the term "naked DNA" was coined by Vical to mean DNA delivered free from agents which promote transfection. Research on the use of naked DNA for DNA vaccination
s and gene therapy
has shown some initial success, but have not yet resulted in any generally available therapy.
Histone
In biology, histones are highly alkaline proteins found in eukaryotic cell nuclei that package and order the DNA into structural units called nucleosomes. They are the chief protein components of chromatin, acting as spools around which DNA winds, and play a role in gene regulation...
-free DNA
DNA
Deoxyribonucleic acid is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms . The DNA segments that carry this genetic information are called genes, but other DNA sequences have structural purposes, or are involved in...
that is passed from cell to cell during a gene transfer process called transformation
Transformation (genetics)
In molecular biology transformation is the genetic alteration of a cell resulting from the direct uptake, incorporation and expression of exogenous genetic material from its surroundings and taken up through the cell membrane. Transformation occurs naturally in some species of bacteria, but it can...
or transfection
Transfection
Transfection is the process of deliberately introducing nucleic acids into cells. The term is used notably for non-viral methods in eukaryotic cells...
. In transformation , purified or naked DNA is taken up by the recipient cell which will give the recipient cell a new characteristic or phenotype
Phenotype
A phenotype is an organism's observable characteristics or traits: such as its morphology, development, biochemical or physiological properties, behavior, and products of behavior...
. Transfection differs from transformation since the DNA is not generally incorporated into the cell's genome, it is only transiently expressed.
In the field of DNA vaccines or genetic immunization, the term "naked DNA" was coined by Vical to mean DNA delivered free from agents which promote transfection. Research on the use of naked DNA for DNA vaccination
DNA vaccination
DNA vaccination is a technique for protecting an organism against disease by injecting it with genetically engineered DNA to produce an immunological response. Nucleic acid vaccines are still experimental, and have been applied to a number of viral, bacterial and parasitic models of disease, as...
s and gene therapy
Gene therapy
Gene therapy is the insertion, alteration, or removal of genes within an individual's cells and biological tissues to treat disease. It is a technique for correcting defective genes that are responsible for disease development...
has shown some initial success, but have not yet resulted in any generally available therapy.
External links
- Naked DNA vaccine research
- DyNAVacS an Integrative Tool for DNA Vaccine Design hosted at the Institute of Genomics and Integrative Biology.