A neurosphere is free-floating structure generated by neural stem cell

Stem cell

This article is about the cell type. For the medical therapy, see Stem Cell TreatmentsStem cells are biological cells found in all multicellular organisms, that can divide and differentiate into diverse specialized cell types and can self-renew to produce more stem cells...

s in vitro

In vitro

In vitro refers to studies in experimental biology that are conducted using components of an organism that have been isolated from their usual biological context in order to permit a more detailed or more convenient analysis than can be done with whole organisms. Colloquially, these experiments...

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Neurospheres present as non-adherent spherical clusters of cells. Many factors contribute to the cellular composition of the neurosphere. Key variables including the age of the animal, plating density, culturing techniques, and passage number will all influence the homogeneity or heterogeneity of the neurosphere.

External links

• A Network Protocol for generating neurospheres has been published on the Nature Protocols
  Nature Protocols
  Nature Protocols, published by the Nature Publishing Group, is an on-line scientific journal publishing methods in a recipe-style format. The journal was launched in June 2006 and the content includes both classical methods and cutting-edge techniques relevant to the study of biological problems...
  
  site: Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation
• Reusable 3D cell culture tool used for growing neurospheres - 3D Petri Dish