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PEAKS (software)
Encyclopedia
PEAKS is a software program for tandem mass spectrometry
designed for peptide
sequencing, protein
identification and quantification.
protein identification (Protein ID), de novo sequencing (both automated and manual), peptide sequence tag
based searching (SPIDER) and quantification (ICAT, iTRAQ, SILAC, Label Free, etc).
PEAKS provides a complete sequence for each peptide, confidence scores on individual amino acid assignments, simple reporting for high-throughput analysis, amongst other information.
The software has the ability to compare results. PEAKS (inChorus) will cross check test results automatically with other protein ID search engines, like Sequest, OMSSA, X!Tandem and Mascot. This approach guards against false positive peptide assignments.
The performance of many mass spectrometry software, including PEAKS, can be observed in the 2011 iPRG study.
Mass spectrometry
Mass spectrometry is an analytical technique that measures the mass-to-charge ratio of charged particles.It is used for determining masses of particles, for determining the elemental composition of a sample or molecule, and for elucidating the chemical structures of molecules, such as peptides and...
designed for peptide
Peptide
Peptides are short polymers of amino acid monomers linked by peptide bonds. They are distinguished from proteins on the basis of size, typically containing less than 50 monomer units. The shortest peptides are dipeptides, consisting of two amino acids joined by a single peptide bond...
sequencing, protein
Protein
Proteins are biochemical compounds consisting of one or more polypeptides typically folded into a globular or fibrous form, facilitating a biological function. A polypeptide is a single linear polymer chain of amino acids bonded together by peptide bonds between the carboxyl and amino groups of...
identification and quantification.
Description
PEAKS is commonly used for search engineSearch engine
A search engine is an information retrieval system designed to help find information stored on a computer system. The search results are usually presented in a list and are commonly called hits. Search engines help to minimize the time required to find information and the amount of information...
protein identification (Protein ID), de novo sequencing (both automated and manual), peptide sequence tag
Peptide sequence tag
A peptide sequence tag is a piece of information about a peptide obtained by tandem mass spectrometry that can be used to identify this peptide in a protein database.-Mass spectrometry:...
based searching (SPIDER) and quantification (ICAT, iTRAQ, SILAC, Label Free, etc).
PEAKS provides a complete sequence for each peptide, confidence scores on individual amino acid assignments, simple reporting for high-throughput analysis, amongst other information.
The software has the ability to compare results. PEAKS (inChorus) will cross check test results automatically with other protein ID search engines, like Sequest, OMSSA, X!Tandem and Mascot. This approach guards against false positive peptide assignments.
The performance of many mass spectrometry software, including PEAKS, can be observed in the 2011 iPRG study.