Para-Nitrophenylphosphate
Encyclopedia
para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid
and alkaline phosphatase
in ELISA
assays. Under their influence the decay to yellow para-nitrophenol
is catalysed. This product can be measured with a 405 nm spectrophotometer.
Acid phosphatase
Acid phosphatase is a phosphatase, a type of enzyme, used to free attached phosphate groups from other molecules during digestion. It is basically a phosphomonoesterase...
and alkaline phosphatase
Alkaline phosphatase
Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation...
in ELISA
ELISA
Enzyme-linked immunosorbent assay , is a popular format of a "wet-lab" type analytic biochemistry assay that uses one sub-type of heterogeneous, solid-phase enzyme immunoassay to detect the presence of a substance in a liquid sample."Wet lab" analytic biochemistry assays involves detection of an...
assays. Under their influence the decay to yellow para-nitrophenol
4-Nitrophenol
4-Nitrophenol is a phenolic compound that has a nitro group at the opposite position of hydroxy group on the benzene ring.-Properties:...
is catalysed. This product can be measured with a 405 nm spectrophotometer.