Primer Premier
Encyclopedia
Primer Premier software combines design of primers for various PCR applications under one common platform. The software supports design of degenerate primers on alignments for amplifying related set of nucleotide sequences for detecting common traits among organisms and to determine heredity.
The software also designs tagged and nested primers for multiplex PCR reactions.
The Primer Premier software supports motif analysis and ORF search along with finding restriction cut sites on the nucleotide sequence.
The software has a built-in nucleotide-protein and protein-nucleotide sequence translator which can be used for designing degenerate primers for codons that code for multiple amino acid sequences. It is also understood that the primers designed by Primer Premier are effective and confirm to the design guidelines laid for designing primers for housekeeping genes with a high G+C%
The software also designs primers for flanking exons and introns in a gene, though there is no mention of it on the official Primer Premier page on the Premier Biosoft
Premier Biosoft
PREMIER Biosoft International is a California, United States based bioinformatics company. The company specializes in developing software for use in life science research...
website.
The software calculates the hairpin structure stability and has been used to study its effect on PCR amplification
Competitors
There are many other software programs that design primers for the PCR reaction. Some of them are listed below:1. Geneious
Geneious
Geneious is suite of cross-platform bioinformatics software applications developed by Biomatters Ltd.- Features :Geneious comes in a Basic version that is free for academic use, and a commercial Pro version with added features. Geneious bundles various bioinformatics tools under one hood with an...
2. Primer 3
3. DNASTAR
DNASTAR
DNASTAR is a bioinformatics company headquartered in Madison, WI that provides software for DNA sequence analysis and assembly, gene expression analysis, and genomic visualization.-History:DNASTAR was incorporated in 1984...
4. AlleleID
5. PrimerX
6. GenomePride
7. OLIGO
OLIGO Primer Analysis Software
OLIGO Primer Analysis Software was the first publicly-available software for DNA primer design. The first papers describing this software were published in 1989 and 1990, and consecutive upgrades in the 1990-s and 2000-s, all have been cited together over 600 times in scientific journals and over...