PstI
Encyclopedia
PstI, is a Type II restriction endonuclease
(or restriction enzyme
) from Providencia stuartii
. PstI recognition and cut site are as follows:
Endonuclease
Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain, in contrast to exonucleases, which cleave phosphodiester bonds at the end of a polynucleotide chain. Typically, a restriction site will be a palindromic sequence four to six nucleotides long. Most...
(or restriction enzyme
Restriction enzyme
A Restriction Enzyme is an enzyme that cuts double-stranded DNA at specific recognition nucleotide sequences known as restriction sites. Such enzymes, found in bacteria and archaea, are thought to have evolved to provide a defense mechanism against invading viruses...
) from Providencia stuartii
Providencia stuartii
Providencia stuartii , is a Gram negative bacterium that is commonly found in soil, water, and sewage. P. stuartii is the most common of the 5 species found in the genus Providencia, with Providencia rettgeri, Providencia alcalifaciens, Providencia rustigianii, P heimbachae...
. PstI recognition and cut site are as follows:
| Recognition Sequence | Cut Site |
|---|---|
5'CTGCAG 3' 3'GACGTC 5' |
5'--CTGCA G--3' 3'--G ACGTC--5' |