Pulse labelling
Encyclopedia
Pulse labelling is a biochemistry
technique of identifying the target molecule
presence by inclusion of a pulse of a radioactive compound. This is mainly done to identify the stage at which the messenger RNA
is being produced in a cell
. In this the pulse of 3H atom is pulsed for a period of around 2 mins to detect the newly formed mRNA during that period.
Biochemistry
Biochemistry, sometimes called biological chemistry, is the study of chemical processes in living organisms, including, but not limited to, living matter. Biochemistry governs all living organisms and living processes...
technique of identifying the target molecule
Molecule
A molecule is an electrically neutral group of at least two atoms held together by covalent chemical bonds. Molecules are distinguished from ions by their electrical charge...
presence by inclusion of a pulse of a radioactive compound. This is mainly done to identify the stage at which the messenger RNA
Messenger RNA
Messenger RNA is a molecule of RNA encoding a chemical "blueprint" for a protein product. mRNA is transcribed from a DNA template, and carries coding information to the sites of protein synthesis: the ribosomes. Here, the nucleic acid polymer is translated into a polymer of amino acids: a protein...
is being produced in a cell
Cell (biology)
The cell is the basic structural and functional unit of all known living organisms. It is the smallest unit of life that is classified as a living thing, and is often called the building block of life. The Alberts text discusses how the "cellular building blocks" move to shape developing embryos....
. In this the pulse of 3H atom is pulsed for a period of around 2 mins to detect the newly formed mRNA during that period.