Purnell equation
Encyclopedia
The Purnell equation is an equation
Equation
An equation is a mathematical statement that asserts the equality of two expressions. In modern notation, this is written by placing the expressions on either side of an equals sign , for examplex + 3 = 5\,asserts that x+3 is equal to 5...

 used in analytical chemistry
Analytical chemistry
Analytical chemistry is the study of the separation, identification, and quantification of the chemical components of natural and artificial materials. Qualitative analysis gives an indication of the identity of the chemical species in the sample and quantitative analysis determines the amount of...

 to calculate the resolution Rs between two peaks in a chromatogram:



where
Rs is the resolution between the two peaks

N2 is the plate number
Plate number
A plate number is a number printed in the margin of a sheet or roll of postage stamps which shows the printing plate used to print the stamps.A plate number is the serial number of a printing plate. It is printed in the selvage or border of a pane of postage stamps. Philatelists and stamp...

 of the second peak

α is the separation factor between the two peaks

k '2 is the retention factor of the second peak.


The higher the resolution, the better the separation.
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