Retention distance
Encyclopedia
Retention distance, or RD, is a concept in thin layer chromatography
, designed for quantitative measurement of equal-spreading of the spots on the chromatographic plate and one of the Chromatographic response function
s. It is calculated from the following formula:
where n is the number of compounds separated, Rf (1...n) are the Retention factor of the compounds sorted in non-descending order, Rf0 = 0 and Rf(n+1) = 1.
This coefficient was proposed as an alternative to earlier approaches, such as delta-Rf, delta-Rf product or MRF (Multispot Response Function). Besides its stable range, the advantage is a stable distribution as a random variable, regardless of compounds investigated.
In contrast to the similar concept called Retention uniformity
, Rd is sensitive to Rf values close to 0 or 1, or close to themselves. If two values are not separated, it is equal to 0. For example the Rf values (0,0.2,0.2,0.3) (two compounds not separated at 0.2 and one at the start ) result in RD equal to 0, but RU equal to 0.3609. When some distance from 0 and spots occurs, the value is larger, for example Rf values (0.1,0.2,0.25,0.3) give RD = 0.4835, RU = 0.4066.
Thin layer chromatography
Thin layer chromatography is a chromatography technique used to separate mixtures. Thin layer chromatography is performed on a sheet of glass, plastic, or aluminum foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose...
, designed for quantitative measurement of equal-spreading of the spots on the chromatographic plate and one of the Chromatographic response function
Chromatographic response function
Chromatographic response function, often abbreviated to CRF is a coefficient which characterize in a quantitative manner, the quality of the separation in the result of a chromatography....
s. It is calculated from the following formula:
where n is the number of compounds separated, Rf (1...n) are the Retention factor of the compounds sorted in non-descending order, Rf0 = 0 and Rf(n+1) = 1.
Theoretical considerations
The coefficient lies always in range <0,1> and 0 indicates worst case of separation (all Rf values equal to 0 or 1), value 1 indicates ideal equal-spreading of the spots, for example (0.25,0.5,0.75) for three solutes, or (0.2,0.4,0.6,0.8) for four solutes.This coefficient was proposed as an alternative to earlier approaches, such as delta-Rf, delta-Rf product or MRF (Multispot Response Function). Besides its stable range, the advantage is a stable distribution as a random variable, regardless of compounds investigated.
In contrast to the similar concept called Retention uniformity
Retention uniformity
Retention uniformity, or RU, is a concept in thin layer chromatography, designed for quantitative measurement of equal-spreading of the spots on the chromatographic plate and one of the Chromatographic response functions...
, Rd is sensitive to Rf values close to 0 or 1, or close to themselves. If two values are not separated, it is equal to 0. For example the Rf values (0,0.2,0.2,0.3) (two compounds not separated at 0.2 and one at the start ) result in RD equal to 0, but RU equal to 0.3609. When some distance from 0 and spots occurs, the value is larger, for example Rf values (0.1,0.2,0.25,0.3) give RD = 0.4835, RU = 0.4066.