Ribotyping
Encyclopedia
Ribotyping involves the fingerprinting
of genomic DNA
restriction fragments
that contain all or part of the genes coding for the 16S
and 23S
rRNA. Conceptually, ribotyping is similar to probing restriction fragments of chromosomal DNA with cloned probes
(randomly cloned probes or probes derived from a specific coding sequence such as that of a virulence factor
).
Genetic fingerprinting
DNA profiling is a technique employed by forensic scientists to assist in the identification of individuals by their respective DNA profiles. DNA profiles are encrypted sets of numbers that reflect a person's DNA makeup, which can also be used as the person's identifier...
of genomic DNA
DNA
Deoxyribonucleic acid is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms . The DNA segments that carry this genetic information are called genes, but other DNA sequences have structural purposes, or are involved in...
restriction fragments
Restriction digest
A restriction digest is a procedure used in molecular biology to prepare DNA for analysis or other processing. It is sometimes termed DNA fragmentation...
that contain all or part of the genes coding for the 16S
16S ribosomal RNA
16S ribosomal RNA is a component of the 30S subunit of prokaryotic ribosomes. It is approximately 1.5kb in length...
and 23S
23S ribosomal RNA
The 23S rRNA is a 2904 nt long component of the large prokaryotic subunit The ribosomal peptidyl transferase activity resides in this rRNA...
rRNA. Conceptually, ribotyping is similar to probing restriction fragments of chromosomal DNA with cloned probes
Hybridization probe
In molecular biology, a hybridization probe is a fragment of DNA or RNA of variable length , which is used in DNA or RNA samples to detect the presence of nucleotide sequences that are complementary to the sequence in the probe...
(randomly cloned probes or probes derived from a specific coding sequence such as that of a virulence factor
Virulence factor
Virulence factors are molecules expressed and secreted by pathogens that enable them to achieve the following:* colonization of a niche in the host...
).