Canada

• British Columbia Highway 401 - Clearbrook-Rosedale section of current British Columbia Highway 1 from 1964 to 1973
• Ontario Highway 401

United States

• U.S. Route 401
  U.S. Route 401
  U.S. Route 401 is an north–south United States highway, a spur of U.S. Route 1, that traverses along the fall line from Sumter, South Carolina to Interstate 85 near Wise, North Carolina.-Route description:...
  
  
• Florida State Road 401
• Georgia State Route 401
• Nevada State Route 401
• New York State Route 401 (former)
• North Carolina Highway 401 (former)
• Pennsylvania Route 401
  Pennsylvania Route 401
  Pennsylvania Route 401 is a short east–west state route in Berks and Chester counties, located in southeast Pennsylvania. The western terminus is at Pennsylvania Route 23 in Caernarvon Township, across the county line from Elverson. The eastern terminus is at U.S...
  
  
• Rhode Island Route 401
  Rhode Island Route 401
  -Route:Route 401 takes the following route through the State:*East Greenwich: ; Route 2 to U.S. 1**Division Street and First Avenue-Notes:Route 401, from just east of Cedar Avenue to First Avenue, runs on the Warwick/East Greenwich town line with Route 401 Eastbound running in East Greenwich and...
  
  
• Virginia State Route 401
  Virginia State Route 401
  State Route 401, also known as part of Van Dorn Street, is a primary state highway in Alexandria, Virginia, United States. It runs from the south city limits near the Van Dorn Street Metro station northeast to State Route 420 , generally paralleling Interstate 395 to the east...
  
  
• Washington State Route 401