SAP1a
Encyclopedia
SAP1A is one of a family of proteins that contains a unique DNA binding domain termed the ETS domain.
SAP1a is a nuclear protein stimulating transcription via the c-fos serum response element, and additionally via an Ets binding site independently of the serum response factor.
Insulin activated the human INSIG2 promoter in a process mediated by phosphorylated SAP1a.
Sap1a is phosphorylated efficiently by ERKs but not by SAPK/JNKs. Serum response factor-dependent ternary complex formation by Sap1a is stimulated by ERK phosphorylation but not by SAPK/JNKs. Moreover, Sap1a-mediated transcription is activated by mitogenic signals but not by cell stress.
ELK1
and SAP1a have been shown to form ternary complexes with SRF
on the serum response elements (SRE) located in the c-fos promoter. ELK1
, SAPla, FLI1
and EWS-FLI1 are able to form ternary complexes with SRF on EGR1 SREs. In addition, ELK1 and SAP1a can also form quarternary complexes on the Egr1 SREI.
Transcription
The transcriptional activation domain of SAP1a resides within the C-terminal region, the function of which may be impeded by the N-terminus. Several potential ERK consensus sites within the C-terminal region of SAP1a can modulate its transactivation efficacy, implicating that SAP1a is a direct target of ERKs.Interactions
SAP1a has been shown to interact with the c-fos serum response element upon recruitment by the serum response factorSAP1a is a nuclear protein stimulating transcription via the c-fos serum response element, and additionally via an Ets binding site independently of the serum response factor.
Insulin activated the human INSIG2 promoter in a process mediated by phosphorylated SAP1a.
Sap1a is phosphorylated efficiently by ERKs but not by SAPK/JNKs. Serum response factor-dependent ternary complex formation by Sap1a is stimulated by ERK phosphorylation but not by SAPK/JNKs. Moreover, Sap1a-mediated transcription is activated by mitogenic signals but not by cell stress.
ELK1
ELK1
E twenty-six -like transcription factor 1, also known as Elk1, functions as a transcription activator. It is classified as a ternary complex factor , a subclass of the ETS family, which is characterized by a common protein domain that regulates DNA binding to target sequences...
and SAP1a have been shown to form ternary complexes with SRF
SRF
SRF may refer to:Name:* SRF Limited, an Indian public company specializing in technical fibers, polymers and engineering plasticsTechnology:* Self-Resonant Frequency, a type resonance in electronic circuit* Server Response File...
on the serum response elements (SRE) located in the c-fos promoter. ELK1
ELK1
E twenty-six -like transcription factor 1, also known as Elk1, functions as a transcription activator. It is classified as a ternary complex factor , a subclass of the ETS family, which is characterized by a common protein domain that regulates DNA binding to target sequences...
, SAPla, FLI1
FLI1
Friend leukemia integration 1 transcription factor , also known as proto-oncogene Fli-1 or transcription factor ERGB, is a protein that in humans is encoded by the human FLI1 gene. - Function :...
and EWS-FLI1 are able to form ternary complexes with SRF on EGR1 SREs. In addition, ELK1 and SAP1a can also form quarternary complexes on the Egr1 SREI.