Saturated mutagenesis
Encyclopedia
Saturation mutagenesis is a form of site-directed mutagenesis
, in which one tries to generate all possible (or as close to as possible) mutations at a specific site, or narrow region of a gene. This is a common technique used in directed evolution
.
Site-directed mutagenesis
Site-directed mutagenesis, also called site-specific mutagenesis or oligonucleotide-directed mutagenesis, is a molecular biology technique in which a mutation is created at a defined site in a DNA molecule. In general, this form of mutagenesis requires that the wild type gene sequence be known...
, in which one tries to generate all possible (or as close to as possible) mutations at a specific site, or narrow region of a gene. This is a common technique used in directed evolution
Directed evolution
thumb|250px|right|An example of a possible round to evolve a protein based fluorescent sensor for a specific analyte using two consecutive FACS sortings...
.