The Expulsion from the Garden of Eden is a fresco

Fresco

Fresco is any of several related mural painting types, executed on plaster on walls or ceilings. The word fresco comes from the Greek word affresca which derives from the Latin word for "fresh". Frescoes first developed in the ancient world and continued to be popular through the Renaissance...

 by the Italian Early Renaissance artist Masaccio

Masaccio

Masaccio , born Tommaso di Ser Giovanni di Simone, was the first great painter of the Quattrocento period of the Italian Renaissance. According to Vasari, Masaccio was the best painter of his generation because of his skill at recreating lifelike figures and movements as well as a convincing sense...

. The fresco is a single scene from the cycle painted around 1425 by Masaccio, Masolino

Masolino da Panicale

Masolino da Panicale was an Italian painter. His best known works are probably his collaborations with Masaccio: Madonna with Child and St. Anne and the frescoes in the Brancacci Chapel .-Biography:Masolino was born in Panicale...

 and others on the walls of the Brancacci Chapel

Brancacci Chapel

The Brancacci Chapel is a chapel in the Church of Santa Maria del Carmine in Florence, central Italy. It is sometimes called the "Sistine Chapel of the early Renaissance" for its painting cycle, among the most famous and influential of the period. Construction of the chapel was commissioned by...

 in the church of Santa Maria del Carmine in Florence

Florence

Florence is the capital city of the Italian region of Tuscany and of the province of Florence. It is the most populous city in Tuscany, with approximately 370,000 inhabitants, expanding to over 1.5 million in the metropolitan area....

. It depicts the expulsion from the garden of Adam and Eve

Adam and Eve

Adam and Eve were, according to the Genesis creation narratives, the first human couple to inhabit Earth, created by YHWH, the God of the ancient Hebrews...

, from the biblical Book of Genesis chapter 3, albeit with a few differences from the canonical account.
Many possible sources of inspiration have been pointed out that Masaccio may have drawn from.