USS Charleston has been the name of more than one United States Navy ship, and may refer to:
, a galley in commission from 1798 to 1802, a protected cruiser commissioned in 1889 and wrecked in 1899, a protected cruiser in commission from 1905 to 1923, a gunboat in commission from 1936 to 1946, an amphibious cargo ship in commission from 1968 to 1992

Fiction

• USS Charleston, a starship
  Starship
  A starship or interstellar spacecraft is a theoretical spacecraft designed for traveling between the stars, as opposed to a vehicle designed for orbital spaceflight or interplanetary travel....
  
   mentioned in the Star Trek: The Next Generation
  Star Trek: The Next Generation
  Star Trek: The Next Generation is an American science fiction television series created by Gene Roddenberry as part of the Star Trek franchise. Roddenberry, Rick Berman, and Michael Piller served as executive producers at different times throughout the production...
  
   episode The Neutral Zone
• USS Charleston (SSN-704), a fictional United States Navy submarine, appears in the 2000 television movie On the Beach (2000 film)
  On the Beach (2000 film)
  On the Beach is an apocalyptic television movie released in 2000, airing originally on Showtime. It is a remake of the 1959 film, and is based on the 1957 novel by Nevil Shute. It starred Armand Assante, Bryan Brown, and Rachel Ward...