ZNF184
Encyclopedia
Zinc finger protein 184, also known as ZNF184, is a protein
that in humans is encoded by the ZNF184 gene
on chromosome 6. It was first identified by Goldwurm et al. in 1996.
The National Center for Biotechnology Information
(NCBI) Gene database entry for ZNF184 identifies conserved domains KRAB_A (Krüppel associated box
) near the N-terminus and Zn-finger (Zinc finger
) at the C-terminus of the expressed protein. The former is associated with transcription repression and the latter with DNA binding (see Zinc finger
).
ZNF184 has 19 zinc finger motifs at the end of its final and longest exon. The figure shows regularity among the fingers in this protein, including the 2 columns of green-highlighted Cys residues and the 2 columns of blue-highlighted His residues which are the reason this type of zinc finger is called C2H2. Light grey highlighted columns (one with all F; one with mostly L, and F substitutions) are highly conserved hydrophobic residues within the zinc finger motif. The other light grey highlighted column (mostly K, with a similar R substitution) is an example of fairly strong conservation in the coil sections connecting adjacent fingers.
Near the N-terminus is a KRAB_A domain followed by a KRAB_B domain. KRAB_A has a shorter α-Helix followed by a longer α-Helix. The KRAB_A motif in a zinc finger protein is known to bind with a KAP-1 protein (aka TRIM28
) to accomplish a transcription repressor function, however a gene so regulated by ZNF184 has yet to be identified. The length-11 finger helices are indicated, as well as the overlapping 7-residue section in each finger which binds targeted DNA (if the finger is functioning).
Protein
Proteins are biochemical compounds consisting of one or more polypeptides typically folded into a globular or fibrous form, facilitating a biological function. A polypeptide is a single linear polymer chain of amino acids bonded together by peptide bonds between the carboxyl and amino groups of...
that in humans is encoded by the ZNF184 gene
Gene
A gene is a molecular unit of heredity of a living organism. It is a name given to some stretches of DNA and RNA that code for a type of protein or for an RNA chain that has a function in the organism. Living beings depend on genes, as they specify all proteins and functional RNA chains...
on chromosome 6. It was first identified by Goldwurm et al. in 1996.
The National Center for Biotechnology Information
National Center for Biotechnology Information
The National Center for Biotechnology Information is part of the United States National Library of Medicine , a branch of the National Institutes of Health. The NCBI is located in Bethesda, Maryland and was founded in 1988 through legislation sponsored by Senator Claude Pepper...
(NCBI) Gene database entry for ZNF184 identifies conserved domains KRAB_A (Krüppel associated box
Krüppel associated box
The Krüppel associated box domain is a category of transcriptional repression domains present in approximately 200 human zinc finger protein-based transcription factors. The KRAB domain typically consists of about 75 amino acid residues whilst the minimal repression module is approximately 45...
) near the N-terminus and Zn-finger (Zinc finger
Zinc finger
Zinc fingers are small protein structural motifs that can coordinate one or more zinc ions to help stabilize their folds. They can be classified into several different structural families and typically function as interaction modules that bind DNA, RNA, proteins, or small molecules...
) at the C-terminus of the expressed protein. The former is associated with transcription repression and the latter with DNA binding (see Zinc finger
Zinc finger
Zinc fingers are small protein structural motifs that can coordinate one or more zinc ions to help stabilize their folds. They can be classified into several different structural families and typically function as interaction modules that bind DNA, RNA, proteins, or small molecules...
).
Domains and Structure
The figure below is a reformatted and annotated protein translation display of ZNF184s Consensus CDS. CCDS displays exons in alternating black and blue font, with red indicating a residue coded across a splice boundary.ZNF184 has 19 zinc finger motifs at the end of its final and longest exon. The figure shows regularity among the fingers in this protein, including the 2 columns of green-highlighted Cys residues and the 2 columns of blue-highlighted His residues which are the reason this type of zinc finger is called C2H2. Light grey highlighted columns (one with all F; one with mostly L, and F substitutions) are highly conserved hydrophobic residues within the zinc finger motif. The other light grey highlighted column (mostly K, with a similar R substitution) is an example of fairly strong conservation in the coil sections connecting adjacent fingers.
Near the N-terminus is a KRAB_A domain followed by a KRAB_B domain. KRAB_A has a shorter α-Helix followed by a longer α-Helix. The KRAB_A motif in a zinc finger protein is known to bind with a KAP-1 protein (aka TRIM28
TRIM28
Tripartite motif-containing 28 also known as transcriptional intermediary factor 1β is a protein that in humans is encoded by the TRIM24 gene.- Function :...
) to accomplish a transcription repressor function, however a gene so regulated by ZNF184 has yet to be identified. The length-11 finger helices are indicated, as well as the overlapping 7-residue section in each finger which binds targeted DNA (if the finger is functioning).