A 16 bit application is any software written for MS-DOS

MS-DOS

MS-DOS is an operating system for x86-based personal computers. It was the most commonly used member of the DOS family of operating systems, and was the main operating system for IBM PC compatible personal computers during the 1980s to the mid 1990s, until it was gradually superseded by operating...

, OS/2

OS/2

OS/2 is a computer operating system, initially created by Microsoft and IBM, then later developed by IBM exclusively. The name stands for "Operating System/2," because it was introduced as part of the same generation change release as IBM's "Personal System/2 " line of second-generation personal...

 1.x or early versions of Microsoft Windows

Microsoft Windows

Microsoft Windows is a series of operating systems produced by Microsoft.Microsoft introduced an operating environment named Windows on November 20, 1985 as an add-on to MS-DOS in response to the growing interest in graphical user interfaces . Microsoft Windows came to dominate the world's personal...

 which originally ran on the 16-bit

16-bit

-16-bit architecture:The HP BPC, introduced in 1975, was the world's first 16-bit microprocessor. Prominent 16-bit processors include the PDP-11, Intel 8086, Intel 80286 and the WDC 65C816. The Intel 8088 was program-compatible with the Intel 8086, and was 16-bit in that its registers were 16...

 Intel 8088

Intel 8088

The Intel 8088 microprocessor was a variant of the Intel 8086 and was introduced on July 1, 1979. It had an 8-bit external data bus instead of the 16-bit bus of the 8086. The 16-bit registers and the one megabyte address range were unchanged, however...

 and Intel 80286

Intel 80286

The Intel 80286 , introduced on 1 February 1982, was a 16-bit x86 microprocessor with 134,000 transistors. Like its contemporary simpler cousin, the 80186, it could correctly execute most software written for the earlier Intel 8086 and 8088...

 microprocessor

Microprocessor

A microprocessor incorporates the functions of a computer's central processing unit on a single integrated circuit, or at most a few integrated circuits. It is a multipurpose, programmable device that accepts digital data as input, processes it according to instructions stored in its memory, and...

s. Such applications used a 20-bit

Bit

A bit is the basic unit of information in computing and telecommunications; it is the amount of information stored by a digital device or other physical system that exists in one of two possible distinct states...

 or 24-bit segment or selector-offset address representation to extend the range of addressable memory locations beyond what was possible using only 16-bit addresses. Programs containing more than byte

Byte

The byte is a unit of digital information in computing and telecommunications that most commonly consists of eight bits. Historically, a byte was the number of bits used to encode a single character of text in a computer and for this reason it is the basic addressable element in many computer...

s (64 kilobyte

Kilobyte

The kilobyte is a multiple of the unit byte for digital information. Although the prefix kilo- means 1000, the term kilobyte and symbol KB have historically been used to refer to either 1024 bytes or 1000 bytes, dependent upon context, in the fields of computer science and information...

s) of instructions and data therefore required special instructions to switch between their 64-kilobyte segment

Memory segment

x86 memory segmentation refers to the implementation of memory segmentation on the x86 architecture. Memory is divided into portions that may be addressed by a single index register without changing a 16-bit segment selector. In real mode or V86 mode, a segment is always 64 kilobytes in size . In...

s, increasing the complexity of programming 16-bit applications.