AlleleID designs oligos for strain differentiation and taxa specific assays. This aids in bacterial identification, pathogen detection and species identification. The software is designed to run on Windows and Macintosh operating system.

AlleleID aligns sequences to locate differences in DNA and to find conserved regions and then designs oligos to amplify and detect only the species or strains of interest from the mix. The platforms for which AlleleID includes support are:

1. qPCR: Designs species specific and taxa specific qPCR assays

2. Microarrays

DNA microarray

A DNA microarray is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome...

: Designs species specific and taxa specific microarrays

3. xMAP®: Designs oligos for Direct Hybridization Assays (DHA) and ASPE assays

4. MLPA

Multiplex ligation-dependent probe amplification

Multiplex ligation-dependent probe amplification is a variation of the polymerase chain reaction that permits multiple targets to be amplified with only a single primer pair. Each probe consists of a two oligonucleotides which recognise adjacent target sites on the DNA...

: Designs probes for copy number detection and SNP studies

External links

• AlleleID Homepage
• PREMIER Biosoft International
• xMAP® Technology
• Introduction to Bacterial Identification