Bacillus subtilis BSR sRNAs
Encyclopedia
In a screen of the Bacillus subtilis
genome for genes encoding ncRNAs, Saito et al. focused on 123 intergenic regions (IGRs) over 500 bp in length, the authors analyzed expression from these regions. Seven IGRs termed bsrC, bsrD, bsrE, bsrF, bsrG, bsrH and bsrI expressed RNAs smaller than 380 nt. All the small RNAs
except BsrD RNA were expressed in transformed Escherichia coli cells harboring a plasmid with PCR-amplified IGRs of B. subtilis, indicating that their own promoters independently express small RNAs. Under non-stressed condition, depletion of the genes for the small RNAs did not affect growth. Although their functions are unknown, gene expression profiles at several time points showed that most of the genes except for bsrD were expressed during the vegetative phase (4–6 h), but undetectable during the stationary phase (8 h). Mapping the 5' ends of the 6 small RNAs revealed that the genes for BsrE, BsrF, BsrG, BsrH, and BsrI RNAs are preceded by a recognition site for RNA polymerase sigma factor σA
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Bacillus subtilis
Bacillus subtilis, known also as the hay bacillus or grass bacillus, is a Gram-positive, catalase-positive bacterium commonly found in soil. A member of the genus Bacillus, B. subtilis is rod-shaped, and has the ability to form a tough, protective endospore, allowing the organism to tolerate...
genome for genes encoding ncRNAs, Saito et al. focused on 123 intergenic regions (IGRs) over 500 bp in length, the authors analyzed expression from these regions. Seven IGRs termed bsrC, bsrD, bsrE, bsrF, bsrG, bsrH and bsrI expressed RNAs smaller than 380 nt. All the small RNAs
Bacterial small RNA
Bacterial small RNAs are small non-coding RNA molecules produced by bacteria, they are highly structured and contain several stem-loops...
except BsrD RNA were expressed in transformed Escherichia coli cells harboring a plasmid with PCR-amplified IGRs of B. subtilis, indicating that their own promoters independently express small RNAs. Under non-stressed condition, depletion of the genes for the small RNAs did not affect growth. Although their functions are unknown, gene expression profiles at several time points showed that most of the genes except for bsrD were expressed during the vegetative phase (4–6 h), but undetectable during the stationary phase (8 h). Mapping the 5' ends of the 6 small RNAs revealed that the genes for BsrE, BsrF, BsrG, BsrH, and BsrI RNAs are preceded by a recognition site for RNA polymerase sigma factor σA
Sigma factor
A sigma factor is a bacterial transcription initiation factor that enables specific binding of RNA polymerase to gene promoters. Different sigma factors are activated in response to different environmental conditions...
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See also
- Bacterial small RNABacterial small RNABacterial small RNAs are small non-coding RNA molecules produced by bacteria, they are highly structured and contain several stem-loops...
- Mycobacterium tuberculosis sRNAMycobacterium tuberculosis sRNAMycobactierum tuberculosis contains at least nine small RNA families in its genome. The small RNA families were identified through RNomics - the direct analysis of RNA molecules isolated from cultures of Mycobacterium tuberculosis. The sRNAs were characterised through RACE mapping and Northern...
- Caenorhabditis elegans small RNAsC. elegans small RNAsSmall RNAs have been identified within the C. elegans genome and comparative genomics has shown that they are conserved across several nematode species. These sRNAs contain a characteristic 2,2,7-trimethylguanosine cap structure that identifies them as non-coding RNAs that have a functional role...
- Escherichia coli sRNAEscherichia coli sRNAEscherichia coli contains a number of small RNAs located in intergenic regions of its genome. The presence of at least 55 of these has been verfied experimentally. 275 potential sRNA-encoding loci were identified computationally using the QRNA program. These loci will include false positives, so...
- Pseudomonas sRNAPseudomonas sRNAPseudomonas sRNA are non-coding RNAs that were predicted by the bioinformatic program SRNApredict2. This program identifies putative sRNAs by searching for co-localization of genetic features commonly associated with sRNA-encoding genes and the expression of the predicted sRNAs was subsequently...