Pseudomonas sRNA are non-coding RNAs

Non-coding RNA

A non-coding RNA is a functional RNA molecule that is not translated into a protein. Less-frequently used synonyms are non-protein-coding RNA , non-messenger RNA and functional RNA . The term small RNA is often used for short bacterial ncRNAs...

 (ncRNA) that were predicted by the bioinformatic

Bioinformatics

Bioinformatics is the application of computer science and information technology to the field of biology and medicine. Bioinformatics deals with algorithms, databases and information systems, web technologies, artificial intelligence and soft computing, information and computation theory, software...

 program SRNApredict2. This program identifies putative sRNAs by searching for co-localization of genetic features commonly associated with sRNA-encoding genes and the expression

Gene expression

Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. These products are often proteins, but in non-protein coding genes such as ribosomal RNA , transfer RNA or small nuclear RNA genes, the product is a functional RNA...

 of the predicted sRNAs was subsequently confirmed by Northern blot

Northern blot

The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA in a sample. With northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression levels during differentiation,...

 analysis. These sRNAs have been shown to be conserved across several pseudomonas

Pseudomonas

Pseudomonas is a genus of gammaproteobacteria, belonging to the family Pseudomonadaceae containing 191 validly described species.Recently, 16S rRNA sequence analysis has redefined the taxonomy of many bacterial species. As a result, the genus Pseudomonas includes strains formerly classified in the...

 species but their function is yet to be determined.

See also

• Mycobacterium tuberculosis sRNA
  Mycobacterium tuberculosis sRNA
  Mycobactierum tuberculosis contains at least nine small RNA families in its genome. The small RNA families were identified through RNomics - the direct analysis of RNA molecules isolated from cultures of Mycobacterium tuberculosis. The sRNAs were characterised through RACE mapping and Northern...
  
  
• Bacillus subtilis sRNA
  Bacillus subtilis BSR sRNAs
  In a screen of the Bacillus subtilis genome for genes encoding ncRNAs, Saito et al. focused on 123 intergenic regions over 500 bp in length, the authors analyzed expression from these regions. Seven IGRs termed bsrC, bsrD, bsrE, bsrF, bsrG, bsrH and bsrI expressed RNAs smaller than 380 nt...
  
  
• Caenorhabditis elegans sRNA
  C. elegans small RNAs
  Small RNAs have been identified within the C. elegans genome and comparative genomics has shown that they are conserved across several nematode species. These sRNAs contain a characteristic 2,2,7-trimethylguanosine cap structure that identifies them as non-coding RNAs that have a functional role...