Collagen gel contraction assay
Encyclopedia
The collagen gel contraction assay is a type of wound contraction
. It is performed using the dermal equivalent
model, which consists of dermal fibroblast
s seeded into a collagen
gel.
Wound healing
Wound healing, or cicatrisation, is an intricate process in which the skin repairs itself after injury. In normal skin, the epidermis and dermis exists in a steady-state equilibrium, forming a protective barrier against the external environment...
. It is performed using the dermal equivalent
Dermal equivalent
The dermal equivalent is an in vitro model of the dermal layer of skin. It is constructed by seeding dermal fibroblasts into a collagen gel. This gel may then be allowed to contract as a model of wound contraction. This collagen gel contraction assay may be used to screen for treatments which...
model, which consists of dermal fibroblast
Dermal fibroblast
Dermal fibroblasts are cells that live within the dermis layer of skin which are responsible for generating connective tissue and allowing the skin to recover from injury. Using organelles , dermal fibroblasts generate and maintain the connective tissue which unites separate cell layers...
s seeded into a collagen
Collagen
Collagen is a group of naturally occurring proteins found in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content...
gel.