IMAGE cDNA clones
Encyclopedia
IMAGE cDNA clones are a collection of DNA vectors
containing cDNAs from various organisms including human
, mouse
, rat
, non-human primates, zebrafish, pufferfish
, Xenopus
(frogs), and cow. http://image.llnl.gov/image/html/iresources.shtml Together they represent a more or less complete set of expressed genes from these organisms. IMAGE stands for integrated molecular analysis of genomes and their expression.
was maintained by the IMAGE Consortium, a joint effort of four academic groups. At the end of 2007, the consortium handed over operations and stocks to a company associated with Open Biosystems.
Cloning vector
A cloning vector is a small piece of DNA into which a foreign DNA fragment can be inserted. The insertion of the fragment into the cloning vector is carried out by treating the vehicle and the foreign DNA with a restriction enzyme that creates the same overhang, then ligating the fragments...
containing cDNAs from various organisms including human
Human
Humans are the only living species in the Homo genus...
, mouse
Mouse
A mouse is a small mammal belonging to the order of rodents. The best known mouse species is the common house mouse . It is also a popular pet. In some places, certain kinds of field mice are also common. This rodent is eaten by large birds such as hawks and eagles...
, rat
Rat
Rats are various medium-sized, long-tailed rodents of the superfamily Muroidea. "True rats" are members of the genus Rattus, the most important of which to humans are the black rat, Rattus rattus, and the brown rat, Rattus norvegicus...
, non-human primates, zebrafish, pufferfish
Pufferfish
Tetraodontidae is a family of primarily marine and estuarine fish of the Tetraodontiformes order. The family includes many familiar species which are variously called pufferfish, balloonfish, blowfish, bubblefish, globefish, swellfish, toadfish, toadies, honey toads, sugar toads, and sea squab...
, Xenopus
Xenopus
Xenopus is a genus of highly aquatic frogs native to Sub-Saharan Africa. There are 19 species in the Xenopus genus...
(frogs), and cow. http://image.llnl.gov/image/html/iresources.shtml Together they represent a more or less complete set of expressed genes from these organisms. IMAGE stands for integrated molecular analysis of genomes and their expression.
Curators
From 1993 to 2007, the cDNA libraryCDNA library
A cDNA library is a combination of cloned cDNA fragments inserted into a collection of host cells, which together constitute some portion of the transcriptome of the organism. cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an...
was maintained by the IMAGE Consortium, a joint effort of four academic groups. At the end of 2007, the consortium handed over operations and stocks to a company associated with Open Biosystems.
See also
- complementary DNAComplementary DNAIn genetics, complementary DNA is DNA synthesized from a messenger RNA template in a reaction catalyzed by the enzyme reverse transcriptase and the enzyme DNA polymerase. cDNA is often used to clone eukaryotic genes in prokaryotes...
(cDNA) - cDNA libraryCDNA libraryA cDNA library is a combination of cloned cDNA fragments inserted into a collection of host cells, which together constitute some portion of the transcriptome of the organism. cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an...
- expressed sequence tags (EST)
- vector (molecular biology)Vector (molecular biology)In molecular biology, a vector is a DNA molecule used as a vehicle to transfer foreign genetic material into another cell. The four major types of vectors are plasmids, viruses, cosmids, and artificial chromosomes...
- cloning vectorCloning vectorA cloning vector is a small piece of DNA into which a foreign DNA fragment can be inserted. The insertion of the fragment into the cloning vector is carried out by treating the vehicle and the foreign DNA with a restriction enzyme that creates the same overhang, then ligating the fragments...