Methylococcus capsulatus
Encyclopedia
Methylococcus capsulatus is an obligately methanotroph
ic gram-negative
, non-motile coccoid bacterium. M. capsulatus cells are encapsulated and tend to have a diplococcoid arrangement. In addition to methane
, M. capsulatus is able to oxidize some organic hydrogen
containing compounds such as methanol
. M. capsulatus has also been demonstrated to be thermotolerant- that is, it can grow well up to 50°C, though its optimum growth temperature is 37°C. In addition, M. capsulatus can live in conditions in which there is little molecular oxygen
available.
Methylococcus capsulatus is a Type I methanotroph, meaning that it is a member of the Gammaproteobacteria
and that it utilizes the ribulose monophosphate pathway (RuMP) for formaldehyde
assimilation
. Methane is first oxidized to methanol
, which then gets converted into formaldehyde. Formaldehyde can then be 1) further oxidized to formate
and carbon dioxide
for energy
production or 2) assimilated into biomass
.
The genome
sequence of M. capsulatus (Bath) is available.
Various strains of M. capsulatus have been isolated and studied, particularly M. capsulatus (Bath), M. capsulatus (Texas) and M. capsulatus (Aberdeen).
Methanotroph
Methanotrophs are bacteria that are able to metabolize methane as their only source of carbon and energy. They can grow aerobically or anaerobically and require single-carbon compounds to survive...
ic gram-negative
Gram-negative
Gram-negative bacteria are bacteria that do not retain crystal violet dye in the Gram staining protocol. In a Gram stain test, a counterstain is added after the crystal violet, coloring all Gram-negative bacteria with a red or pink color...
, non-motile coccoid bacterium. M. capsulatus cells are encapsulated and tend to have a diplococcoid arrangement. In addition to methane
Methane
Methane is a chemical compound with the chemical formula . It is the simplest alkane, the principal component of natural gas, and probably the most abundant organic compound on earth. The relative abundance of methane makes it an attractive fuel...
, M. capsulatus is able to oxidize some organic hydrogen
Hydrogen
Hydrogen is the chemical element with atomic number 1. It is represented by the symbol H. With an average atomic weight of , hydrogen is the lightest and most abundant chemical element, constituting roughly 75% of the Universe's chemical elemental mass. Stars in the main sequence are mainly...
containing compounds such as methanol
Methanol
Methanol, also known as methyl alcohol, wood alcohol, wood naphtha or wood spirits, is a chemical with the formula CH3OH . It is the simplest alcohol, and is a light, volatile, colorless, flammable liquid with a distinctive odor very similar to, but slightly sweeter than, ethanol...
. M. capsulatus has also been demonstrated to be thermotolerant- that is, it can grow well up to 50°C, though its optimum growth temperature is 37°C. In addition, M. capsulatus can live in conditions in which there is little molecular oxygen
Oxygen
Oxygen is the element with atomic number 8 and represented by the symbol O. Its name derives from the Greek roots ὀξύς and -γενής , because at the time of naming, it was mistakenly thought that all acids required oxygen in their composition...
available.
Methylococcus capsulatus is a Type I methanotroph, meaning that it is a member of the Gammaproteobacteria
Gammaproteobacteria
Gammaproteobacteria is a class of several medically, ecologically and scientifically important groups of bacteria, such as the Enterobacteriaceae , Vibrionaceae and Pseudomonadaceae. An exceeding number of important pathogens belongs to this class, e.g...
and that it utilizes the ribulose monophosphate pathway (RuMP) for formaldehyde
Formaldehyde
Formaldehyde is an organic compound with the formula CH2O. It is the simplest aldehyde, hence its systematic name methanal.Formaldehyde is a colorless gas with a characteristic pungent odor. It is an important precursor to many other chemical compounds, especially for polymers...
assimilation
Cultural assimilation
Cultural assimilation is a socio-political response to demographic multi-ethnicity that supports or promotes the assimilation of ethnic minorities into the dominant culture. The term assimilation is often used with regard to immigrants and various ethnic groups who have settled in a new land. New...
. Methane is first oxidized to methanol
Methanol
Methanol, also known as methyl alcohol, wood alcohol, wood naphtha or wood spirits, is a chemical with the formula CH3OH . It is the simplest alcohol, and is a light, volatile, colorless, flammable liquid with a distinctive odor very similar to, but slightly sweeter than, ethanol...
, which then gets converted into formaldehyde. Formaldehyde can then be 1) further oxidized to formate
Formate
Formate or methanoate is the ion CHOO− or HCOO− . It is the simplest carboxylate anion. It is produced in large amounts in the hepatic mitochondria of embryonic cells and in cancer cells by the folate cycle Formate or methanoate is the ion CHOO− or HCOO− (formic acid minus one hydrogen ion). It...
and carbon dioxide
Carbon dioxide
Carbon dioxide is a naturally occurring chemical compound composed of two oxygen atoms covalently bonded to a single carbon atom...
for energy
Energy
In physics, energy is an indirectly observed quantity. It is often understood as the ability a physical system has to do work on other physical systems...
production or 2) assimilated into biomass
Biomass
Biomass, as a renewable energy source, is biological material from living, or recently living organisms. As an energy source, biomass can either be used directly, or converted into other energy products such as biofuel....
.
The genome
Genome
In modern molecular biology and genetics, the genome is the entirety of an organism's hereditary information. It is encoded either in DNA or, for many types of virus, in RNA. The genome includes both the genes and the non-coding sequences of the DNA/RNA....
sequence of M. capsulatus (Bath) is available.
Various strains of M. capsulatus have been isolated and studied, particularly M. capsulatus (Bath), M. capsulatus (Texas) and M. capsulatus (Aberdeen).