PrimerPlex designs oligos for standard multiplex PCR assays and for bead-based suspension array systems such as BioPlex-200 and Luminex 100/200 based on xMAP technology. The assays are used for high-throughput SNP applications such as genotyping, pathogen detection, strain typing, and haplotyping. It is supported on both Windows and Mac.

The following oligo design options are supported:

1. Multiplex PCR Primer Design: Can design highly specific multiplex primers for standard PCR assays for up to 30 sequences.
2. Direct Hybridization Assay (DHA) Oligo Design: Can design probes for 100 individual targets in a multiplex reaction. The SNPs are located at the center to maximize discrimination.
3. ASPE Assay Oligo Design: Can design primers for 40 individual targets in a multiplex reaction. The mutation is located at the 3' end.

The author company, PREMIER Biosoft

Premier Biosoft

PREMIER Biosoft International is a California, United States based bioinformatics company. The company specializes in developing software for use in life science research...

, has supported strain differentiation multiplex xMAP assays in one of their other products, AlleleID

AlleleID

AlleleID designs oligos for strain differentiation and taxa specific assays. This aids in bacterial identification, pathogen detection and species identification...

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External links

• PrimerPlex Home Page
• Multiplex PCR: An Overview