Radiation reduced hybrid
Encyclopedia
Radiation reduced hybrid is procedure in discovering the location of genetic markers relative to one another. The relative location of these markers can be combined into a physical map
or a genetic map. The radiation hybrid technique begins as another way to amplify and purify DNA
, the first step in any sequencing project; radiation is used to break the DNA into pieces and these pieces are incorporated into the hybrid cell; the hybrid can be grown in large quantities. One can then check for the presence of various genetic markers using PCR and linkage analysis to resolve the distance between the markers. That is, if two genetic markers are near each other, they are less likely to be separated by the DNA-breaking radiation. The technique is similar to traditional linkage analysis, where we depend on genetic recombination
to calculate the distance between two genetic markers.
but contain genes that that can resist the toxin when contained by the same cell. The cell line under study is irradiated, causing breaks in the DNA. These cells are fused with the other cell line, producing a hybrid. If the hybrid incorporates genes from both cells, it will be able to survive in the toxic media.
The cells that survive can be grown in large quantities, thus amplifying the DNA that was incorporated from the irradiated cell line. One can prepare a sample of DNA from the hybrid cell line and use PCR to amplify two specific genetic markers. By running the PCR products on a gel, one can determine if both markers are in the cell line. If both markers are usually in the hybrid, we can conclude that they are near each other.
Physical map
Physical map may refer to:* Physical map , maps that shows countries of the world.* Physical map , showing how much DNA separates two genes and is measured in base pairs, as opposed to a genetic map...
or a genetic map. The radiation hybrid technique begins as another way to amplify and purify DNA
DNA
Deoxyribonucleic acid is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms . The DNA segments that carry this genetic information are called genes, but other DNA sequences have structural purposes, or are involved in...
, the first step in any sequencing project; radiation is used to break the DNA into pieces and these pieces are incorporated into the hybrid cell; the hybrid can be grown in large quantities. One can then check for the presence of various genetic markers using PCR and linkage analysis to resolve the distance between the markers. That is, if two genetic markers are near each other, they are less likely to be separated by the DNA-breaking radiation. The technique is similar to traditional linkage analysis, where we depend on genetic recombination
Genetic recombination
Genetic recombination is a process by which a molecule of nucleic acid is broken and then joined to a different one. Recombination can occur between similar molecules of DNA, as in homologous recombination, or dissimilar molecules, as in non-homologous end joining. Recombination is a common method...
to calculate the distance between two genetic markers.
Procedure
The procedure utilizes two cell lines, neither of which can survive in toxic media,but contain genes that that can resist the toxin when contained by the same cell. The cell line under study is irradiated, causing breaks in the DNA. These cells are fused with the other cell line, producing a hybrid. If the hybrid incorporates genes from both cells, it will be able to survive in the toxic media.
The cells that survive can be grown in large quantities, thus amplifying the DNA that was incorporated from the irradiated cell line. One can prepare a sample of DNA from the hybrid cell line and use PCR to amplify two specific genetic markers. By running the PCR products on a gel, one can determine if both markers are in the cell line. If both markers are usually in the hybrid, we can conclude that they are near each other.