A teloblast is a large cell in the embryos of clitellate annelid

Annelid

The annelids , formally called Annelida , are a large phylum of segmented worms, with over 17,000 modern species including ragworms, earthworms and leeches...

s which asymmetrically divide to form many smaller cells known as blast cells. These blast cells further proliferate and differentiate to form the segmental

Segmentation (biology)

Segmentation in biology refers to either a type of gastrointestinal motility or the division of some animal and plant body plans into a series of repetitive segments. This article will focus on the segmentation of animal body plans, specifically using the examples of the phyla Arthropoda,...

 tissues of the annelid. Teloblasts are well studied in leeches, though they are also present in the other major class of clitellates: the oligochaetes.

Developmental Role / Morphology

All teloblasts are specified from the D quadrant macromere after the second round of divisions post-fertilization. There are five pairs of teloblasts, one on each side of the embryo. Four of the teloblasts (N, O, P, and Q) give rise to ectodermal tissue and one pair (M) gives rise to mesodermal tissue. The column of blast cells arising out of each teloblast is known as a bandlet. All five bandlets coalesce into one germinal band on each side of the embryo, extending out from the teloblast towards the head (in the rostral direction). The teloblasts are located at the rear of the embryo.

Teloblasts have two separate cytoplasmic domains: the teloplasm and the vitelloplasm. The teloplasm contains the nucleus

Cell nucleus

In cell biology, the nucleus is a membrane-enclosed organelle found in eukaryotic cells. It contains most of the cell's genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. The genes within these...

, ribosomes, mitochondria, and other subcellular organelles. The vitelloplasm contains mostly yolk platelets. Only the teloplasm gets passed onto the daughter stem cells after cell division

Cell division

Cell division is the process by which a parent cell divides into two or more daughter cells . Cell division is usually a small segment of a larger cell cycle. This type of cell division in eukaryotes is known as mitosis, and leaves the daughter cell capable of dividing again. The corresponding sort...

.

O/P specification

The O and P teloblasts are specified from two separate but identical precursors, which form an equivalence group These two precursor cells are termed O/P cells for their ability to become either O or P teloblasts. Signals from the surrounding cells act to specify which fate the teloblasts and their progeny take on. Interactions with the q bandlet, however transient, can induce the p fate in the adjacent o/p bandlet . The M bandlet has been shown to In some species (i.e. Helobdella triserialis), the provisional epithelium covering the cells plays a role in inducing the O fate . In the absence of cell-cell interactions, the O/P precusors will become O teloblasts. O and P bandlets exhibit very different mitotic patterns (see figure) which are used to identify them in experimental manipulations.

Segmental fates

The N and Q teloblasts contribute two blast cells per segment, one making up the anterior half of the segment, the second making up the posterior half of the segment. The O, P, and M lineages contribute one blast cell per segment, but the contributions from each blast cell spans a segmental boundary. These segmental boundaries were discovered by injecting teloblasts with cell lineage tracers after a few blast cells have already been generated. During development, the N and Q bandlets, which eventually have 64 blast cells each, slide past the O,P,and M bandlet, which only have 32 cells. Thus, the segmental boundaries within each bandlet are already specified before all the bandlets come into complete register .