The bead test is a traditional part of qualitative inorganic analysis

Qualitative inorganic analysis

Classical qualitative inorganic analysis is a method of analytical chemistry which seeks to find elemental composition of inorganic compounds. It is mainly focused on detecting ions in an aqueous solution, so that materials in other forms may need to be brought into this state before using standard...

 to test for the presence of certain metals. The oldest one is the borax bead test or blister test. It was introduced by Berzelius

Berzelius

Berzelius is a secret society at Yale University named for the Swedish scientist Jöns Jakob Berzelius, considered one of the founding fathers of modern chemistry...

 in 1812. Since then other salts were used as fluxing agents, such as sodium carbonate

Sodium carbonate

Sodium carbonate , Na2CO3 is a sodium salt of carbonic acid. It most commonly occurs as a crystalline heptahydrate, which readily effloresces to form a white powder, the monohydrate. Sodium carbonate is domestically well-known for its everyday use as a water softener. It can be extracted from the...

 or sodium fluoride

Sodium fluoride

Sodium fluoride is an inorganic chemical compound with the formula NaF. A colorless solid, it is a source of the fluoride ion in diverse applications. Sodium fluoride is less expensive and less hygroscopic than the related salt potassium fluoride....

. The most important one after borax is microcosmic salt

Microcosmic salt

Microcosmic salt is a salt found in urine with the formula NaHPO4. In the mineral form, microcosmic salt is called stercorite....

, which is the basis of the microcosmic salt bead test.

Borax bead test

A small loop is made in the end of a platinum

Platinum

Platinum is a chemical element with the chemical symbol Pt and an atomic number of 78. Its name is derived from the Spanish term platina del Pinto, which is literally translated into "little silver of the Pinto River." It is a dense, malleable, ductile, precious, gray-white transition metal...

 wire (as used in the flame test

Flame test

A flame test is a procedure used in chemistry to detect the presence of certain metal ions, based on each element's characteristic emission spectrum. The color of flames in general also depends on temperature; see flame color....

) and heated in a Bunsen

Bunsen

Bunsen may refer to:* Christian Bunsen , chief librarian of Göttingen and professor of modern philology, father of Robert Bunsen* Christian Charles Josias Bunsen , Prussian diplomat and scholar...

 flame until red hot. It is then dipped into powdered borax

Borax

Borax, also known as sodium borate, sodium tetraborate, or disodium tetraborate, is an important boron compound, a mineral, and a salt of boric acid. It is usually a white powder consisting of soft colorless crystals that dissolve easily in water.Borax has a wide variety of uses...

, and the adhering solid is held in the hottest part of the flame where it swells up as it loses its water of crystallization

Water of crystallization

In crystallography, water of crystallization or water of hydration or crystallization water is water that occurs in crystals. Water of crystallization is necessary for the maintenance of crystalline properties, but capable of being removed by sufficient heat...

 and then shrinks, forming a colourless, transparent glass-like bead (a mixture of sodium metaborate and boric anhydride),

The bead is moistened (traditionally with the tongue) and dipped into the sample to be tested such that only a tiny amount of the substance adheres to the bead. If too much substance is used, the bead will become dark and opaque. The bead and adhering substance is then heated in the lower, reducing, part of the flame, allowed to cool, and the colour observed. It is then heated in the upper, oxidizing, part of the flame, allowed to cool, and the colour observed again.

Characteristic coloured beads are produced with salts of copper

Copper

Copper is a chemical element with the symbol Cu and atomic number 29. It is a ductile metal with very high thermal and electrical conductivity. Pure copper is soft and malleable; an exposed surface has a reddish-orange tarnish...

, iron

Iron

Iron is a chemical element with the symbol Fe and atomic number 26. It is a metal in the first transition series. It is the most common element forming the planet Earth as a whole, forming much of Earth's outer and inner core. It is the fourth most common element in the Earth's crust...

, chromium

Chromium

Chromium is a chemical element which has the symbol Cr and atomic number 24. It is the first element in Group 6. It is a steely-gray, lustrous, hard metal that takes a high polish and has a high melting point. It is also odorless, tasteless, and malleable...

, manganese

Manganese

Manganese is a chemical element, designated by the symbol Mn. It has the atomic number 25. It is found as a free element in nature , and in many minerals...

, cobalt

Cobalt

Cobalt is a chemical element with symbol Co and atomic number 27. It is found naturally only in chemically combined form. The free element, produced by reductive smelting, is a hard, lustrous, silver-gray metal....

 and nickel

Nickel

Nickel is a chemical element with the chemical symbol Ni and atomic number 28. It is a silvery-white lustrous metal with a slight golden tinge. Nickel belongs to the transition metals and is hard and ductile...

. After the test, the bead is removed by heating it to fusion point, and plunging it into a vessel of water.

Metal Metal A metal , is an element, compound, or alloy that is a good conductor of both electricity and heat. Metals are usually malleable and shiny, that is they reflect most of incident light...	Oxidising flame	Reducing flame
Aluminum	colorless (hot and cold), opaque	colorless, opaque
Antimony Antimony Antimony is a toxic chemical element with the symbol Sb and an atomic number of 51. A lustrous grey metalloid, it is found in nature mainly as the sulfide mineral stibnite...	colorless, yellow or brown (hot)	gray and opaque
Barium Barium Barium is a chemical element with the symbol Ba and atomic number 56. It is the fifth element in Group 2, a soft silvery metallic alkaline earth metal. Barium is never found in nature in its pure form due to its reactivity with air. Its oxide is historically known as baryta but it reacts with...	colorless
Bismuth Bismuth Bismuth is a chemical element with symbol Bi and atomic number 83. Bismuth, a trivalent poor metal, chemically resembles arsenic and antimony. Elemental bismuth may occur naturally uncombined, although its sulfide and oxide form important commercial ores. The free element is 86% as dense as lead...	colorless, yellow or brownish (hot)	gray and opaque
Cadmium Cadmium Cadmium is a chemical element with the symbol Cd and atomic number 48. This soft, bluish-white metal is chemically similar to the two other stable metals in group 12, zinc and mercury. Similar to zinc, it prefers oxidation state +2 in most of its compounds and similar to mercury it shows a low...	colorless	gray and opaque
Calcium Calcium Calcium is the chemical element with the symbol Ca and atomic number 20. It has an atomic mass of 40.078 amu. Calcium is a soft gray alkaline earth metal, and is the fifth-most-abundant element by mass in the Earth's crust...	colorless
Cerium Cerium Cerium is a chemical element with the symbol Ce and atomic number 58. It is a soft, silvery, ductile metal which easily oxidizes in air. Cerium was named after the dwarf planet . Cerium is the most abundant of the rare earth elements, making up about 0.0046% of the Earth's crust by weight...	red (hot)	colorless (hot and cold)
Chromium Chromium Chromium is a chemical element which has the symbol Cr and atomic number 24. It is the first element in Group 6. It is a steely-gray, lustrous, hard metal that takes a high polish and has a high melting point. It is also odorless, tasteless, and malleable...	Dark yellow (hot), green (cold)	green (hot and cold)
Cobalt Cobalt Cobalt is a chemical element with symbol Co and atomic number 27. It is found naturally only in chemically combined form. The free element, produced by reductive smelting, is a hard, lustrous, silver-gray metal....	blue (hot and cold)	blue (hot and cold)
Copper Copper Copper is a chemical element with the symbol Cu and atomic number 29. It is a ductile metal with very high thermal and electrical conductivity. Pure copper is soft and malleable; an exposed surface has a reddish-orange tarnish...	green (hot), blue (cold)	red, opaque (cold), colorless (hot)
Iron Iron Iron is a chemical element with the symbol Fe and atomic number 26. It is a metal in the first transition series. It is the most common element forming the planet Earth as a whole, forming much of Earth's outer and inner core. It is the fourth most common element in the Earth's crust...	yellow or brownish red (hot and cold)	green (hot and cold)
Lead Lead Lead is a main-group element in the carbon group with the symbol Pb and atomic number 82. Lead is a soft, malleable poor metal. It is also counted as one of the heavy metals. Metallic lead has a bluish-white color after being freshly cut, but it soon tarnishes to a dull grayish color when exposed...	colorless, yellow or brownish (hot)	gray and opaque
Magnesium Magnesium Magnesium is a chemical element with the symbol Mg, atomic number 12, and common oxidation number +2. It is an alkaline earth metal and the eighth most abundant element in the Earth's crust and ninth in the known universe as a whole...	colorless
Manganese Manganese Manganese is a chemical element, designated by the symbol Mn. It has the atomic number 25. It is found as a free element in nature , and in many minerals...	|violet (hot and cold)	colorless (hot and cold)
Molybdenum Molybdenum Molybdenum , is a Group 6 chemical element with the symbol Mo and atomic number 42. The name is from Neo-Latin Molybdaenum, from Ancient Greek , meaning lead, itself proposed as a loanword from Anatolian Luvian and Lydian languages, since its ores were confused with lead ores...	colorless	yellow or brown (hot)
Nickel Nickel Nickel is a chemical element with the chemical symbol Ni and atomic number 28. It is a silvery-white lustrous metal with a slight golden tinge. Nickel belongs to the transition metals and is hard and ductile...	brown, red (cold)	gray and opaque (cold)
Silicon Silicon Silicon is a chemical element with the symbol Si and atomic number 14. A tetravalent metalloid, it is less reactive than its chemical analog carbon, the nonmetal directly above it in the periodic table, but more reactive than germanium, the metalloid directly below it in the table...	colorless (hot and cold), opaque	colorless, opaque
Silver Silver Silver is a metallic chemical element with the chemical symbol Ag and atomic number 47. A soft, white, lustrous transition metal, it has the highest electrical conductivity of any element and the highest thermal conductivity of any metal...	colorless	gray and opaque
Strontium Strontium Strontium is a chemical element with the symbol Sr and the atomic number 38. An alkaline earth metal, strontium is a soft silver-white or yellowish metallic element that is highly reactive chemically. The metal turns yellow when exposed to air. It occurs naturally in the minerals celestine and...	colorless
Tin Tin Tin is a chemical element with the symbol Sn and atomic number 50. It is a main group metal in group 14 of the periodic table. Tin shows chemical similarity to both neighboring group 14 elements, germanium and lead and has two possible oxidation states, +2 and the slightly more stable +4...	colorless (hot and cold), opaque	colorless, opaque
Titanium Titanium Titanium is a chemical element with the symbol Ti and atomic number 22. It has a low density and is a strong, lustrous, corrosion-resistant transition metal with a silver color....	colorless	yellow (hot), violet (cold)
Tungsten Tungsten Tungsten , also known as wolfram , is a chemical element with the chemical symbol W and atomic number 74.A hard, rare metal under standard conditions when uncombined, tungsten is found naturally on Earth only in chemical compounds. It was identified as a new element in 1781, and first isolated as...	colorless	brown
Uranium Uranium Uranium is a silvery-white metallic chemical element in the actinide series of the periodic table, with atomic number 92. It is assigned the chemical symbol U. A uranium atom has 92 protons and 92 electrons, of which 6 are valence electrons...	Yellow or brownish (hot)	green
Vanadium Vanadium Vanadium is a chemical element with the symbol V and atomic number 23. It is a hard, silvery gray, ductile and malleable transition metal. The formation of an oxide layer stabilizes the metal against oxidation. The element is found only in chemically combined form in nature...	colorless	green